In:
FEBS Letters, Wiley, Vol. 423, No. 3 ( 1998-02-27), p. 362-366
Abstract:
The collecting duct epithelium originates from the embryonic ureter by branching morphogenesis. Ontogeny‐dependent changes of CFTR mRNA expression were assessed by quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) in primary monolayer cultures of rat ureteric buds (UB) and cortical collecting ducts, microdissected at different embryonic and postnatal developmental stages. The amount of wild‐type CFTR‐specific PCR product in UB declined to 20% of the initial value between embryonic gestational day E15 and postnatal day P1. After birth the CFTR product increased transiently between P1 and P7 by a factor of 10 and decreased towards day P14. PCR products specific for TRN‐CFTR, a truncated splice variant, however, were low in early embryonic cells, increased markedly between day E17 and P2, and reached a plateau postnatally. Therefore, mRNA encoding TRN‐CFTR does not appear to have a specific embryonic‐morphogenetic function. By contrast, such function is suggested for wild‐type CFTR mRNA as its abundance was high in early embryonic nephrogenesis, as well as during a postnatal period shortly before branching morphogenesis is completed.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(98)00112-4
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
1460391-3
SSG:
12
Bookmarklink
