In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 14 ( 2023-9-20)
Abstract:
Members of the archaeal order Caldarchaeales (previously the phylum Aigarchaeota) are poorly sampled and are represented in public databases by relatively few genomes. Additional representative genomes will help resolve their placement among all known members of Archaea and provide insights into their roles in the environment. In this study, we analyzed 16S rRNA gene amplicons belonging to the Caldarchaeales that are available in public databases, which demonstrated that archaea of the order Caldarchaeales are diverse, widespread, and most abundant in geothermal habitats. We also constructed five metagenome-assembled genomes (MAGs) of Caldarchaeales from two geothermal features to investigate their metabolic potential and phylogenomic position in the domain Archaea . Two of the MAGs were assembled from microbial community DNA extracted from fumarolic lava rocks from Mauna Ulu, Hawai‘i, and three were assembled from DNA obtained from hot spring sinters from the El Tatio geothermal field in Chile. MAGs from Hawai‘i are high quality bins with completeness & gt;95% and contamination & lt;1%, and one likely belongs to a novel species in a new genus recently discovered at a submarine volcano off New Zealand. MAGs from Chile have lower completeness levels ranging from 27 to 70%. Gene content of the MAGs revealed that these members of Caldarchaeales are likely metabolically versatile and exhibit the potential for both chemoorganotrophic and chemolithotrophic lifestyles. The wide array of metabolic capabilities exhibited by these members of Caldarchaeales might help them thrive under diverse harsh environmental conditions. All the MAGs except one from Chile harbor putative prophage regions encoding several auxiliary metabolic genes (AMGs) that may confer a fitness advantage on their Caldarchaeales hosts by increasing their metabolic potential and make them better adapted to new environmental conditions. Phylogenomic analysis of the five MAGs and over 3,000 representative archaeal genomes showed the order Caldarchaeales forms a monophyletic group that is sister to the clade comprising the orders Geothermarchaeales (previously Candidatus Geothermarchaeota), Conexivisphaerales and Nitrososphaerales (formerly known as Thaumarchaeota), supporting the status of Caldarchaeales members as a clade distinct from the Thaumarchaeota.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2023.1216591
DOI:
10.3389/fmicb.2023.1216591.s001
DOI:
10.3389/fmicb.2023.1216591.s002
DOI:
10.3389/fmicb.2023.1216591.s003
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10.3389/fmicb.2023.1216591.s004
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10.3389/fmicb.2023.1216591.s005
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10.3389/fmicb.2023.1216591.s006
DOI:
10.3389/fmicb.2023.1216591.s007
DOI:
10.3389/fmicb.2023.1216591.s008
DOI:
10.3389/fmicb.2023.1216591.s009
DOI:
10.3389/fmicb.2023.1216591.s010
DOI:
10.3389/fmicb.2023.1216591.s011
DOI:
10.3389/fmicb.2023.1216591.s012
DOI:
10.3389/fmicb.2023.1216591.s013
DOI:
10.3389/fmicb.2023.1216591.s014
DOI:
10.3389/fmicb.2023.1216591.s015
DOI:
10.3389/fmicb.2023.1216591.s016
DOI:
10.3389/fmicb.2023.1216591.s017
DOI:
10.3389/fmicb.2023.1216591.s018
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2587354-4
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