In:
Photochemistry and Photobiology, Wiley, Vol. 47, No. 4 ( 1988-04), p. 523-525
Abstract:
Abstract— A Xenon‐chloride excimer laser emitting energy at 308 nm was used to induce single‐strand breaks (SSBs, frank breaks plus alkali‐labile lesions as assayed by alkaline sucrose sedimentation techniques) in purified DNA from Bacillus subtilis . A fluence response study and a peak pulse intensity study were performed. At a pulse energy of 0.1 mJ/pulse, the radiation induced SSBs in a linear fashion (91 SSB/10 8 Da per MJ/m 2 ) to a maximum exprimental fluence of 1.28 MJ/m 2 . The pulse intensity study showed that there were no significant changes in DNA breakage (105 SSB/10 8 Da) between 2.93 times 10 9 and 5.86 times 10 11 W/m 2 (0.11 and 22.0 mJ/pulse) at a constant total fluence of 1.1 MJ/m 2 (27000 mJ dose). This study has verified and extended previous work by quantifying the yield of SSBs induced in DNA by this laser radiation.
Type of Medium:
Online Resource
ISSN:
0031-8655
,
1751-1097
DOI:
10.1111/php.1988.47.issue-4
DOI:
10.1111/j.1751-1097.1988.tb08839.x
Language:
English
Publisher:
Wiley
Publication Date:
1988
detail.hit.zdb_id:
123540-0
detail.hit.zdb_id:
2048860-9
SSG:
12
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