In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 79, No. 23 ( 1982-12), p. 7445-7449
Abstract:
A new tester strain, TA102, is described as an addition to the set of strains for the Salmonella/microsome mutagenicity test. This strain contains A X T base pairs at the site of the mutation (determined by DNA sequence analysis) in contrast to the other Salmonella tester strains that detect mutagens damaging G X C base pairs. This strain differs from previous tester strains in that the mutation has been introduced into a multicopy plasmid, so that approximately equal to 30 copies of the mutant gene are available for back mutation. The new strain detects a variety of oxidative mutagens, including x-rays, bleomycin, hydrogen peroxide and other hydroperoxides, streptonigrin and other quinones, and phenylhydrazine; a variety of aldehydes, including formaldehyde, glyoxal, kethoxal, glutaraldehyde, and malondialdehyde; a number of psoralens (in the presence of near-UV light), mitomycin C, neocarzinostatin, and UV light. Some of these mutagens have been previously shown to damage thymine in DNA. Several auxiliary tester strains also are described, including TA96, a frameshift tester strain with a hot spot for mutation at a run of five A X T base pairs with a specificity similar to that of TA102. The importance of oxidative mutagens is discussed.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.79.23.7445
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
1982
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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