In:
British Journal of Haematology, Wiley, Vol. 102, No. 4 ( 1998-09), p. 1081-1089
Abstract:
In this study we report on the establishment and characterization of two novel lymphoma cell lines (CRO‐AP/3 and CRO‐AP/5) which carry infection by human herpesvirus type‐8 (HHV‐8) and have derived from AIDS‐related primary effusion lymphoma (PEL). These two cell lines are representative of different virologic subtypes of PEL, i.e. HHV‐8 + /EBV − PEL in the case of CRO‐AP/3 and HHV‐8 + /EBV + PEL in the case of CRO‐AP/5. Consistent with the diagnosis of PEL, both CRO‐AP/3 and CRO‐AP/5 expressed indeterminate (i.e. non‐B, non‐T) phenotypes although immunogenotypic studies documented their B‐cell origin. Both cell lines are devoid of genetic lesions of c‐ MYC , BCL ‐2 and p53 as well as gross rearrangements of BCL ‐6. Detailed histogenetic characterization of these novel PEL cell lines suggests that PEL may derive from a post‐germinal centre B cell which has undergone pre‐terminal differentiation. The CRO‐AP/3 and CRO‐AP/5 cell lines may provide a valuable model for clarifying the pathogenesis of PEL. In particular, these cell lines may help understand the relative contribution of HHV‐8 and EBV to PEL growth and development and may facilitate the identification of recurrent cytogenetic abnormalities highlighting putative novel cancer related loci relevant to PEL.
Type of Medium:
Online Resource
ISSN:
0007-1048
,
1365-2141
DOI:
10.1046/j.1365-2141.1998.00877.x
Language:
English
Publisher:
Wiley
Publication Date:
1998
detail.hit.zdb_id:
1475751-5
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