In:
Chemistry – A European Journal, Wiley, Vol. 23, No. 21 ( 2017-04-11), p. 5005-5009
Abstract:
d ‐Fructose‐6‐phosphate aldolase (FSA) was probed for extended nucleophile promiscuity by using a series of fluorogenic substrates to reveal retro‐aldol activity. Four nucleophiles ethanal, propanone, butanone, and cyclopentanone were subsequently confirmed to be non‐natural substrates in the synthesis direction using the wild‐type enzyme and its D6H variant. This exceptional widening of the nucleophile substrate scope offers a rapid entry, in good yields and high stereoselectivity, to less oxygenated alkyl ketones and aldehydes, which was hitherto impossible.
Type of Medium:
Online Resource
ISSN:
0947-6539
,
1521-3765
DOI:
10.1002/chem.201701020
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
1478547-X
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