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  • 1
    Online Resource
    Online Resource
    Tomato rugose mosaic virus in Tomato Crops in São Paulo State, Brazil
    FapUNIFESP (SciELO) ; 2006
    In:  Fitopatologia Brasileira Vol. 31, No. 6 ( 2006-12), p. 606-606
    Details
    In: Fitopatologia Brasileira, FapUNIFESP (SciELO), Vol. 31, No. 6 ( 2006-12), p. 606-606
    Type of Medium: Online Resource
    ISSN: 0100-4158
    URL: Article
    DOI: 10.1590/S0100-41582006000600015
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2006
    detail.hit.zdb_id: 2078802-2
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  • 2
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    Online Resource
    Reação de progênies de pimentão ao Potato Virus Y
    FapUNIFESP (SciELO) ; 2003
    In:  Bragantia Vol. 62, No. 1 ( 2003), p. 53-60
    Details
    In: Bragantia, FapUNIFESP (SciELO), Vol. 62, No. 1 ( 2003), p. 53-60
    Abstract: This research aimed to investigate reaction of sweet pepper (Capsicum annuum L.) progenies to an isolate of Potato virus Y (PVY) from cv. Magda, from Paulínia, State of São Paulo, Brazil, and to identify this isolate. The identification was made by biological tests, using host plants, and by serological tests (TAS-ELISA) employing monoclonal antibodies against the PVY0, PVY N and PVY C strains. Thirty five progenies, from the IAC improvement program, were inoculated with this isolate, named PVY-Pa, and five commercial cultivars whose reaction to PVY was known were used as positive control. In these experiments a completely randomized design was used. Biological tests and electron microscopy showed a mixed infection by potyvirus and tobamovirus in the original samples of sweet pepper. The PVY was isolated from Nicotiana glutinosa leaves with systemic symptoms and in ultra-thin sections, cylindrical cytoplasmic inclusions like pinwheels, were recognized. Such cytopathic effect is unique for the family Potyviridae. In TAS-ELISA infected samples of N. glutinosa reacted negatively against the monoclonal antibodies employed. However, the absence of symptoms on 'Myr 10' and 'Myr 29'Capsicum cultivars was associated with PVYm strain. Resistance of the sweet pepper progenies was assessed through the ratio between the absence and presence of symptoms. From such progenies, seven F3 three-way hybrid progenies showed assymptomatic plants from which the virus could not be recovered by inoculation on N. glutinosa, thus confirming that the virus does not multiply in those progenies.
    Type of Medium: Online Resource
    ISSN: 0006-8705
    URL: Article
    DOI: 10.1590/S0006-87052003000100007
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2003
    detail.hit.zdb_id: 2016147-5
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  • 3
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    Avaliação da resistência de acessos de tomateiro a tospovírus e a geminivírus
    FapUNIFESP (SciELO) ; 2004
    In:  Horticultura Brasileira Vol. 22, No. 2 ( 2004-06), p. 193-196
    Details
    In: Horticultura Brasileira, FapUNIFESP (SciELO), Vol. 22, No. 2 ( 2004-06), p. 193-196
    Abstract: Thirteen lines and three commercial tomato cultivars were screened for tospovirus and geminivirus resistance under field conditions, in Campinas, São Paulo State, Brazil. The germplasm comprised advanced IAC lines plus cultivars Stevens, Franco and IPA-5. The same experiment was carried out in two planting seasons. In the first (December 2001 to February 2002), only Tomato chlorotic spot virus (TCSV) occurred in the field. In the second (July to September 2002), Tomato yellow vein streak virus (TYVSV) was identified in the experimental area, and the evaluation was carried out through counting of infected plants and by symptom intensity scoring by notes. There were no treatment differences by using the first criterion, but the score evaluation was effective in detecting differences among genotypes; line IAC-S3-16 showed an outstanding performance. Considering the two trials, the susceptibility of 'IPA-5' to both pathogens was confirmed; in addition, resistance of 'Franco' to TCSV and TYVSV was observed. Lines IAC-S3-54, IAC-S3-318, IAC-S4-39 and IAC-SVS-1 could be distinguished for the low infection by TCSV. So, these lines can be considered as promising genetic material to obtain cultivars with resistance to both viruses and good agronomic traits.
    Type of Medium: Online Resource
    ISSN: 0102-0536
    URL: Article
    DOI: 10.1590/S0102-05362004000200006
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2004
    detail.hit.zdb_id: 2125666-4
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  • 4
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    Online Resource
    First occurrence of Dasheen mosaic virus (DsMV) in Xanthosoma riedelianum (Mangarito) in Brazil
    Revista Mexicana de Fitopatologia, Mexican Journal of Phytopathology ; 2023
    In:  Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology Vol. 41, No. 2 ( 2023-04-29)
    Details
    In: Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology, Revista Mexicana de Fitopatologia, Mexican Journal of Phytopathology, Vol. 41, No. 2 ( 2023-04-29)
    Abstract: Xantosoma riedelianum, also called “mangarito” in Brazil, is an unconventional food plant (UFP) of the Araceae family native to tropical America. Foliar samples of the X. riedelianum showing mosaic, mottle and vein clearing symptoms were collected in the municipality of Monte Alegre (São Paulo State). Transmission electron microscope observations, mechanical transmission to experimental plants, serological (DAS-ELISA) and RT-PCR analyzes indicated that the symptoms could be associated with the presence of dasheen mosaic virus (DsMV, Potyvirus). This is the first report of DsMV in X. riedelianum in Brazil.
    Type of Medium: Online Resource
    ISSN: 2007-8080
    URL: Journal
    URL: Article
    DOI: 10.18781/R.MEX.FIT.
    DOI: 10.18781/R.MEX.FIT.2303-3
    Language: Unknown
    Publisher: Revista Mexicana de Fitopatologia, Mexican Journal of Phytopathology
    Publication Date: 2023
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  • 5
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    First report of Rhizoctonia solani Kühn AG 2-2 LP in Zoyzia japonica Steud in Brazil
    FapUNIFESP (SciELO) ; 2020
    In:  Summa Phytopathologica Vol. 46, No. 4 ( 2020-12), p. 289-298
    Details
    In: Summa Phytopathologica, FapUNIFESP (SciELO), Vol. 46, No. 4 ( 2020-12), p. 289-298
    Abstract: RESUMO O uso de gramas cultivadas no Brasil cresceu 40% entre 2010 e 2015, sendo que a espécie Zoysia japonica Steud, principalmente a cultivar ‘Esmeralda’, corresponde a 81% do mercado de gramas cultivadas no país. A doença mais importante da grama zoysia, conhecida por “large patch”, é causada por Rhizoctonia solani, ocorrendo nos gramados brasileiros especialmente nos meses de inverno. O objetivo deste trabalho foi contribuir para a identificação e caracterização do grupo de anastomose de isolados de R. solani provenientes de lesões típicas de “large patch” coletados em grama esmeralda de jardins e campos de golfe nos estados de São Paulo e Bahia, Brasil. Os 12 isolados obtidos apresentaram colônias marrom escuro, com crescimento micelial aéreo, hifas multinucleadas, e ausência de zonação concêntrica ou escleródios, bem como exibiram sua maior taxa de crescimento micelial a 25°C. Em experimentos de patogenicidade, os isolados de R. solani, com exceção de três, reduziram o crescimento das plantas de grama zoysia. Baseado na análise das sequencias da região ITS do rDNA, os isolados agruparam com isolados referências do grupo de anastomose AG 2-2 LP. Inferências filogenéticas mostraram que os isolados brasileiros estão agrupados em dois clados que compartilham o mesmo ancestral comum com 96% booststrap. Em um dos clados estão apenas isolados brasileiros e no outro estão também isolados de R. solani AG 2-2 LP americanos e japoneses. Este é o primeiro relato e caracterização de R. solani AG 2-2 LP em grama zoysia no Brasil.
    Type of Medium: Online Resource
    ISSN: 1980-5454 , 0100-5405
    URL: Article
    DOI: 10.1590/0100-5405/221916
    Language: English
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2020
    detail.hit.zdb_id: 2256664-8
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  • 6
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    Online Resource
    Avaliação da reação de genótipos de alface (Lactuca sativa L.) ao Lettuce mosaic virus (LMV)
    FapUNIFESP (SciELO) ; 2007
    In:  Bragantia Vol. 66, No. 1 ( 2007), p. 61-68
    Details
    In: Bragantia, FapUNIFESP (SciELO), Vol. 66, No. 1 ( 2007), p. 61-68
    Abstract: Reactions to one isolate from diseased lettuce plants collected in 2003 in Atibaia, SP, were studied in six varieties and 18 selected lettuce (Lactuca sativa L.) lines. Underdeveloped, malformed leaves with mosaic symptoms and also necrotic leaves were found under field conditions in Raider, 'Karla H25' and Hortência lettuce cultivars. These symptomatic plants were sampled and the virus etiology studied by PTA-ELISA and by means of mechanical inoculation on test plants and on differential lettuce cultivars resistant to Lettuce mosaic virus, LMV pathotypes I, II and III. LMV pathotype IV was identified in all cultivars studied by the reaction in the differential hosts. 'Karla H25'isolate was selected to screen the best lines of the breeding as well as lettuce program and commercial varieties. Twenty-four best lines of the program were inoculated with this isolate and the evaluations were based on symptoms. Symptomless lettuce plants were additionally tested for LMV presence, in PTA-ELISA, and by retro-inoculation to Chenopodium amaranticolor. The results indicated lines with varied degrees of susceptibility and tolerance. Lines 3 and 4 showed tolerance and this suggests that they can provide a good alternative for disease control and also that they might contribute in the reduction of chemical control.
    Type of Medium: Online Resource
    ISSN: 0006-8705
    URL: Article
    DOI: 10.1590/S0006-87052007000100008
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2007
    detail.hit.zdb_id: 2016147-5
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  • 7
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    Online Resource
    Reação de acessos de Lycopersicon spp. a um isolado de Potato Virus Y (PVYº) de tomateiro
    FapUNIFESP (SciELO) ; 2008
    In:  Bragantia Vol. 67, No. 2 ( 2008), p. 391-399
    Details
    In: Bragantia, FapUNIFESP (SciELO), Vol. 67, No. 2 ( 2008), p. 391-399
    Abstract: Samples of tomato (Lycopersicon esculentum Mill) ‘Alambra’ were collected in commercial fields of Elias Fausto, Monte-Mor and Mogi-Guaçu, SP. The plants with virus-like symptoms, especially yellow mosaic were submitted, to biological and serological identification tests. For biological tests, the host range was determined by indicator and differential plants in the family Chenopodiaceae and Solanaceae. The serological identification was made by PTA-ELISA with polyclonal antiserum against Tospovirus, Tobamovirus, Potyvirus and Cucumovirus and by DAS-ELISA, by using monoclonal antibodies against PVY common strain (PVYº), PVY chlorotic strain (PVY C) and PVY necrotic strain (PVY N). All the tested samples showed positive reaction in PTA-ELISA with antiserum against PVY and negative reaction with the antiserum against the remaining viruses. In DAS-ELISA, positive reaction occurred with PVY common strain. Among the host plants, Chenopodium amaranticolor reacted with local lesions, while N. glutinosa and N. tabacum ‘WB’ showed systemic mosaic and the ‘Alambra’ reacted with yellow mosaic, while Datura stramonium, D. metel and C. annuum ‘Magda’ were not infected. The reaction on the differential host C. annuum ‘Magda’ permitted to identify the PVY pathotype 1 in all the 19 samples. To evaluate the reaction of the 19 accesses of Lycopersicon spp. from the IAC active bank of germoplasm, one of the samples of tomato ‘Alambra’ from Elias Fausto was inoculated on tomato accesses and also, the variety ‘Alambra’ was used as a positive control. The experiments delineation was randomized and the plant reaction was evaluated by presence and type of symptoms, and also by positive and negative results after back inoculation and serological tests (PTA-ELISA), and by analysis of χ2 using the ratios between symptomatic and asymptomatic plants with positive or negative results in ELISA. It was verified that most evaluated accesses were tolerant to PVYº-1, but the wild species L. peruvianum B, LA-371-2 and radiated 1C had a great number of asymptomatic plants with negative results by ELISA, constituting potential sources of genes of resistance against PVY in tomatoes.
    Type of Medium: Online Resource
    ISSN: 0006-8705
    URL: Article
    DOI: 10.1590/S0006-87052008000200015
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2008
    detail.hit.zdb_id: 2016147-5
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  • 8
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    Online Resource
    Indexação do Lettuce mosaic virus em sementes e plântulas de genótipos de alface
    FapUNIFESP (SciELO) ; 2012
    In:  Revista Brasileira de Sementes Vol. 34, No. 4 ( 2012), p. 628-635
    Details
    In: Revista Brasileira de Sementes, FapUNIFESP (SciELO), Vol. 34, No. 4 ( 2012), p. 628-635
    Abstract: Lettuce mosaic virus is the major virus in lettuce (Lactuca sativa L.) in Brazil, causing symptoms of mosaic, vein clearing, necrosis, leaf distortion and growth reduction. The LMV is transmitted by seeds at a rate of 1% to 16%, mostly due to interaction between the isolates LMV-Most and LMV-Common and the lettuce cultivar. In this study, LMV detection by PTA-ELISA in seeds and seedlings of eight lettuce genotypes, 'Vanessa Roxa', 'Baba de Verão', 'Verdinha', 'Maravilha das 4 Estações', 'Evely', 'Marcela', 687 ('Vera' X 'Sapore'), 784 ('Vera' X 'Sapore'), was evaluated, using specific polyclonal antisera. The virus was not detected in seeds of 'Verdinha' genotype and seedlings of 687, 'Marcela' and 'Evely' after germination in a paper towel or in 687, 784 and 'Marcela' with the mo1¹ resistance gene, after germination in a substrate. The individual evaluation of the number of infected seeds made by PTA-ELISA was 100% for 'Vanessa Roxa' and 'Baba de Verão', 87.7% for 'Verdinha', 46.6% for 'Maravilha das 4 Estações' and 16.6% for 'Evely'. In the genotypes with the resistance gene the percentage was 15.6%, 26.6%, 90% in 'Marcela', 687 and 784, respectively. Virus detection in seeds and seedlings by PTA-ELISA was very efficient for both seeds and seedlings.
    Type of Medium: Online Resource
    ISSN: 0101-3122
    URL: Article
    DOI: 10.1590/S0101-31222012000400014
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2012
    detail.hit.zdb_id: 2223451-2
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  • 9
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    Online Resource
    Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil
    FapUNIFESP (SciELO) ; 2005
    In:  Fitopatologia Brasileira Vol. 30, No. 4 ( 2005-08), p. 416-419
    Details
    In: Fitopatologia Brasileira, FapUNIFESP (SciELO), Vol. 30, No. 4 ( 2005-08), p. 416-419
    Abstract: Em levantamentos realizados entre 1998 e 2003, nas principais regiões produtoras de alface (Lactuca sativa) e escarola (Cichorium endivia) no cinturão verde de São Paulo, foram observados sintomas de espessamento de nervuras foliares, clorose, crescimento irregular e ausência de formação da cabeça. Por meio de testes biológicos, DAS-Enzyme linked immunosorbent assay (Elisa) e microscopia eletrônica de transmissão constatou-se a presença do Lettuce big-vein associated virus e Mirafiori lettuce virus, responsáveis pela síndrome do espessamento clorótico das nervuras da alface ("lettuce big-vein").
    Type of Medium: Online Resource
    ISSN: 0100-4158
    URL: Article
    DOI: 10.1590/S0100-41582005000400014
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2005
    detail.hit.zdb_id: 2078802-2
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  • 10
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    Online Resource
    Resistência de cultivares e linhagens de tomateiro a Tomato chlorotic spot virus e a Potato virus Y
    FapUNIFESP (SciELO) ; 2005
    In:  Fitopatologia Brasileira Vol. 30, No. 6 ( 2005-12), p. 609-614
    Details
    In: Fitopatologia Brasileira, FapUNIFESP (SciELO), Vol. 30, No. 6 ( 2005-12), p. 609-614
    Abstract: Advanced breeding lines from the IAC tomato breeding program and several tomato (Lycopersicon esculentum) cultivars and F1 hybrids were screened for tospovirus and potyvirus resistance under field conditions, at Campinas, São Paulo State, Brazil, during the 2002/2003 and 2003/2004 growing seasons. During the first season, only Tomato chlorotic spot virus (TCSV) was detected in plants of the experimental area. On both evaluations, all seven lines (IAC group) were resistant to TCSV, with under 28% of infected plants. The tomato cultivars and hybrids were highly susceptible, with greater than 85% of infected plants, except for 'Franco', with 55% of infected plants. During the 2003/2004 growing season, the number of IAC lines evaluated was raised from seven to ten, and they were compared to five cultivars/F1 hybrids and to L. peruvianum LA-444-1. On this experiment, only Potato virus Y (PVY) was detected in plants. Evaluations were carried out using a symptom intensity scale and ELISA. Considering both criteria, it was verified that only LA-444-1 displayed high resistance to PVY. In addition, 'Tyrade' displayed an intermediate behavior while all other lines, cultivars and hybrids behaved as susceptible to this potyvirus. These results highlight the need of introgressing resistance to multiple viruses in tomato breeding programs, taking into consideration the economical importance and relative incidence of each virus in different geographical regions and natural variations on incidence from year to year.
    Type of Medium: Online Resource
    ISSN: 0100-4158
    URL: Article
    DOI: 10.1590/S0100-41582005000600007
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2005
    detail.hit.zdb_id: 2078802-2
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