In:
Blood, American Society of Hematology, Vol. 128, No. 22 ( 2016-12-02), p. 3380-3380
Abstract:
BACKGROUND: Peripheral blood (PB) hematopoietic progenitor cells (HPC) mobilized with G-CSF are the first-choice source for allogeneic stem cell transplantation. We carried out a prospective study on healthy donors (HDs), to identify donor characteristics that could influence the effectiveness of mobilization. STUDY DESIGN AND METHODS: PB-HPC allogeneic donations from sibling HDs were analyzed. We tested somatic variables (sex, age, weight, height, volemia) and blood counts (WBC, platelets, hemoglobin, CD34+ cell count). Two different determinations of CD34+ cells were done in each HD: baseline (before G-CSF administration) and in PB on the morning of the fifth day (after G-CSF administration). HDs received G-CSF subcutaneously at a dose of 10 µg/kg per day. RESULTS: 128 consecutive HDs (66 males) with a median age of 43 years were enrolled. The mean value of CD34+ on day 5 was 90.8 cells/µL, 84.2 cells/µL in females and 97.2 cells/µL in males. The median values of CD34+ on day 5 were 75.5 cells/µL for the overall sample, 74 cells/µL in females and 80 cells/µL in males. On univariate correlation analysis, donor weight r=0.19, P 〈 0.05, height (r=0.20, P 〈 0.05), volemia (r=0.18, P 〈 0.05) and baseline CD34+ (r=0.38, P 〈 0.001) were significant predictors of CD34+ on day 5. Data analysis by gender showed that CD34+ on day 5 were predicted by age (r=-0.30, P 〈 0.05), baseline WBC (r=0.25, P 〈 0.05), baseline CD34+ (r=0.31, P 〈 0.05) in males and by baseline CD34+ (r=0.45, P 〈 0.001) in females. We performed two multivariate analyses either by using ordinary regression (to predict the mean value of CD34+ on day 5) or median regression (to predict the median value of CD34+ on day 5) according to the values of CD34+ at baseline (see table) in the whole study population and separately in females. These results indicate that in the whole study population and by using ordinary regression, a baseline value of CD34+ ranging from 0.5 to 2 cells/µL predicts a mean CD34+ value of 75.7 cells/µL at 5 days whereas a baseline value of CD34+ ≥3 cells/µL predicts a mean CD34+ value of 107.5 cells/µL at 5 days. Furthermore, in the whole study population but by using the median regression, a baseline value of CD34+ ranging from 0.5 to 2 cells/µL predicts a median CD34 + value of 64.6 cells/µL on day 5 whereas a baseline value of CD34+≥3 predicts a median CD34 value of 101.1 cells/µL on day 5. CONCLUSIONS: Baseline, HDs PB CD34+ cell counts correlated with the effectiveness of allogeneic HPC mobilization and could be useful to plan the collection. Disclosures No relevant conflicts of interest to declare.
Type of Medium:
Online Resource
ISSN:
0006-4971
,
1528-0020
DOI:
10.1182/blood.V128.22.3380.3380
Language:
English
Publisher:
American Society of Hematology
Publication Date:
2016
detail.hit.zdb_id:
1468538-3
detail.hit.zdb_id:
80069-7
Bookmarklink