In:
Visual Neuroscience, Cambridge University Press (CUP), Vol. 18, No. 4 ( 2001-07), p. 571-580
Abstract:
Retinal neurotransmitters are known to play a role in postnatal
ocular development and eye growth. The success of muscarinic antagonists in blocking form-deprivation myopia has implicated
retinal acetylcholine in the control of ocular growth. The present study investigated whether steady-state content of acetylcholine
(ACh) and its metabolite choline (Ch) are altered in the retina
of eyes developing axial myopia, in both tree shrews and chicks. Retinal ACh and Ch content were measured using reverse-phase
high-performance liquid chromatography. Posterior and anterior retinal samples were analyzed from myopic (form deprived) and
control eyes as well as age-matched normal eyes. Normative data on retinal neurotransmitter content demonstrated that chick
retinas contained less than half the ACh and Ch neurotransmitter content of tree shrews when normalized to retinal protein (ACh:
61 ± 3 vs . 130 ± 6 ng, Ch: 131 ±
5 vs . 347 ± 25 ng). There was no significant
difference in either ACh or Ch content between myopic and contralateral control eyes in either tree shrews or chicks,
irrespective of the degree of myopia. This finding was consistent for both posterior, anterior, and consequently whole retinal
samples. In contrast, dopamine and DOPAC contents were found to be reduced in myopic compared to control eyes of the same
tree shrews (dopamine −6.9% and DOPAC −15.5%) and chicks (dopamine −12.3% and DOPAC −28.2%). These
findings demonstrate that, contrary to dopamine and DOPAC content, steady-state retinal acetylcholine and choline content is not
significantly altered during myopia development.
Type of Medium:
Online Resource
ISSN:
0952-5238
,
1469-8714
DOI:
10.1017/S0952523801184075
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2001
detail.hit.zdb_id:
1489922-X
SSG:
12
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