In:
Environmental Science: Nano, Royal Society of Chemistry (RSC), Vol. 8, No. 10 ( 2021), p. 2811-2823
Abstract:
Protein adsorption onto mineral nanoparticle surfaces is critical to the function and fate of biological compounds in environmental and industrial systems. However, adsorption kinetics, coverage, and conformation of biological macromolecules are poorly understood, particularly in the presence of ubiquitous oxyanions. In this study, the adsorption of two proteins, beta-lactoglobulin (β-LG) and bovine serum albumin (BSA), onto hematite (α-Fe 2 O 3 ) nanoparticles was investigated in the presence and absence of pre-adsorbed phosphate. Using solution and temporal solid-phase attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, our results show dynamic changes in the secondary structure of both proteins when adsorbed onto nanoscale α-Fe 2 O 3 surfaces, compared to their unbound conformations. However, these differences were attenuated in the presence of adsorbed phosphate. Adsorbed phosphate significantly reduced the protein surface coverage on iron oxide nanoparticle surfaces, and impacted protein adsorption kinetics. The latter was observed to be protein-specific, with β-LG exhibiting a higher adsorption rate and sigmoidal kinetics compared to slower, more Langmuir-type kinetics of BSA adsorption. Our results reveal the importance of phosphate on protein–mineral adsorption kinetics and conformation, a critical driver of protein function, in complex environmental systems.
Type of Medium:
Online Resource
ISSN:
2051-8153
,
2051-8161
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2021
detail.hit.zdb_id:
2758235-8
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