In:
Journal of Biomaterials Applications, SAGE Publications, Vol. 29, No. 1 ( 2014-07), p. 59-71
Abstract:
To improve bone engineering for clinical applications, we coupled nanofiber-peptide hydrogel to nano-hydroxyapatite/collagen to form a bioactive scaffold (cnHAC) that mimics extracellular matrices. In comparison to nano-hydroxyapatite/collagen, we found that cnHAC promoted cell adhesion and spreading, and DNA content measurements, alkaline phosphatase activity assays, and reverse transcriptase-polymerase chain reaction analyses of osteogenic gene expression showed that cnHAC significantly improved cellular attachment, proliferation, and osteogenic differentiation in vitro ( P 〈 0.05). In vivo models based on rat calvarial implants showed that cnHAC significantly enhanced bone regeneration ( P 〈 0.05). In conclusion, we demonstrated that novel cnHAC scaffolds could potentially facilitate future bone regenerative medicine.
Type of Medium:
Online Resource
ISSN:
0885-3282
,
1530-8022
DOI:
10.1177/0885328213514467
Language:
English
Publisher:
SAGE Publications
Publication Date:
2014
detail.hit.zdb_id:
2072559-0
SSG:
12
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