In:
Cytometry, Wiley, Vol. 6, No. 3 ( 1985-05), p. 219-225
Abstract:
Human lymphocytes, transferred in culture into medium conditioned with phytohemaglutinin, were studied with the combined use of quantitative 14 C‐uridine autoradiography and high‐resolution image analysis. During the first 24 h before the onset of DNA synthesis it was possible to study pure G 0 and G 1 populations. The return of the proliferating cells into the G 0 state was clearly monitored in an RNA synthesis rate/DNA content plot during 1‐week growth. On days 3–6, the proliferation decreased progressively, and more cells accumulated in the quiescent G 0 state. Geometric, densitometric, and textural parameters evaluated from the Feulgenstained nuclei of the same cells indicated that the decondensation/condensation processes of the nuclear chromatin during the G 0 °CG 1 transitions paralleled the RNA synthesis activity. The results help to associate descriptors of nuclear morphology, as derived from image analysis, with functional parameters during activation and return to quiescence of human lymphocytes.
Type of Medium:
Online Resource
ISSN:
0196-4763
,
1097-0320
DOI:
10.1002/cyto.990060308
Language:
English
Publisher:
Wiley
Publication Date:
1985
detail.hit.zdb_id:
2180639-1
detail.hit.zdb_id:
1474272-X
detail.hit.zdb_id:
2180651-2
SSG:
12
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