In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_11 ( 2013-11-01), p. 4149-4157
Abstract:
A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01 T stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01 T was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C 15 : 0 , iso-C 16 : 0 , anteiso-C 17 : 0 and iso-C 17 : 0 . The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym- homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01 T represents the first reported cultivated member of subdivision 23 of the Acidobacteria . It is proposed that strain MP-01 T represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01 T ( = DSM 24856 T = JCM 18256 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.051425-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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