In:
Transfusion Medicine and Hemotherapy, S. Karger AG, Vol. 45, No. 4 ( 2018), p. 252-257
Abstract:
〈 b 〉 Background: 〈 /b 〉 Molecular typing for 〈 i 〉 RHCE 〈 /i 〉 blood group alleles has been established in many countries for patients and blood donors. In the Chinese literature nearly 80% of transfused patients with alloimmunization have antibodies specific for antigens of the Rh blood group system. We investigated if it is feasible to match packed red blood cells (RBCs) for Chinese β-thalassemia patients by 〈 i 〉 RHCE 〈 /i 〉 genotyping. 〈 b 〉 Methods: 〈 /b 〉 In this study, 481 patients with β-thalassemia were enrolled. They were genotyped for 〈 i 〉 RHCE 〈 /i 〉 alleles by a simple PCR method with sequence-specific primers (PCR-SSP). Among these patients, 203 continuously received RBCs of the identical Rh subgroups according to the genotyping results for at least 3 months. Subsequently, their phenotypes were tested through a micro-column gel card method. For validation purposes, 400 donors were serologically typed with the same technology, of which 164 were genotyped too. Finally, the C, c, E, and e frequencies and the feasibility of the simple genotyping method were analyzed. 〈 b 〉 Results: 〈 /b 〉 All patients showed mixed-field agglutination in the Rh subgroup gel cards before the same Rh subgroups in blood donors were selected for blood transfusion. The results, however, lacked mixed-field agglutination in all 203 cases after transfusion with RBC concentrates selected for the patient's C, c, E, and e antigens for at least 3 months. The genotyping results of 164 donors were all consistent with the serological results. Whole coding regions of 〈 i 〉 RHCE 〈 /i 〉 were sequenced in 7 individuals with weak c, E, or e antigens. In only one sample we observed a 1059G 〉 A nucleotide mutation coding for a truncated RhCE polypeptide (GenBank KT957625), in the other 6 samples no sequence variant was found. Both patients and donors were predominantly CcEe and CCee, with a prevalence of 55.3% and 24.9% for patients or 49.3% and 31.3% for donors, respectively. It revealed that about 80% of Chinese could receive Rh-matched RBCs easily. 〈 b 〉 Conclusion: 〈 /b 〉 A simple 〈 i 〉 RHCE 〈 /i 〉 genotyping technique is safe enough for Rh-matched transfusion of β-thalassemia patients in Chinese Han.
Type of Medium:
Online Resource
ISSN:
1660-3796
,
1660-3818
Language:
English
Publisher:
S. Karger AG
Publication Date:
2018
detail.hit.zdb_id:
2100533-3
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