In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 6 ( 2023-6-8), p. e1011425-
Abstract:
Cryptosporidium hominis is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the availability of optimized/standardized singular sources of infectious parasite oocysts for research and human challenge studies. The human C . hominis TU502 isolate is currently propagated in gnotobiotic piglets in only one laboratory, which limits access to oocysts. Streamlined cryopreservation could enable creation of a biobank to serve as an oocyst source for research and distribution to other investigators requiring C . hominis . Here, we report cryopreservation of C . hominis TU502 oocysts by vitrification using specially designed specimen containers scaled to 100 μL volume. Thawed oocysts exhibit ~70% viability with robust excystation and 100% infection rate in gnotobiotic piglets. The availability of optimized/standardized sources of oocysts may streamline drug and vaccine evaluation by enabling wider access to biological specimens.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011425
DOI:
10.1371/journal.ppat.1011425.g001
DOI:
10.1371/journal.ppat.1011425.g002
DOI:
10.1371/journal.ppat.1011425.g003
DOI:
10.1371/journal.ppat.1011425.g004
DOI:
10.1371/journal.ppat.1011425.g005
DOI:
10.1371/journal.ppat.1011425.g006
DOI:
10.1371/journal.ppat.1011425.s001
DOI:
10.1371/journal.ppat.1011425.s002
DOI:
10.1371/journal.ppat.1011425.s003
DOI:
10.1371/journal.ppat.1011425.s004
DOI:
10.1371/journal.ppat.1011425.s005
DOI:
10.1371/journal.ppat.1011425.s006
DOI:
10.1371/journal.ppat.1011425.s007
DOI:
10.1371/journal.ppat.1011425.s008
DOI:
10.1371/journal.ppat.1011425.s009
DOI:
10.1371/journal.ppat.1011425.s010
DOI:
10.1371/journal.ppat.1011425.s011
DOI:
10.1371/journal.ppat.1011425.s012
DOI:
10.1371/journal.ppat.1011425.s013
DOI:
10.1371/journal.ppat.1011425.s014
DOI:
10.1371/journal.ppat.1011425.s015
DOI:
10.1371/journal.ppat.1011425.r001
DOI:
10.1371/journal.ppat.1011425.r002
DOI:
10.1371/journal.ppat.1011425.r003
DOI:
10.1371/journal.ppat.1011425.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2205412-1
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