In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 11 ( 2020-11-19), p. e0242058-
Abstract:
The tumor immune microenvironment is emerging as a critical player in predicting cancer prognosis and response to therapies. However, the prognostic value of tumor-infiltrating immune cells in Gingivo-Buccal Oral Squamous Cell Carcinoma (GBOSCC) and their association with tumor size or lymph node metastases status require further elucidation. To study the relationship of tumor-infiltrating immune cells with tumor size (T stage) and lymph node metastases (N stages), we analyzed the density of tumor-infiltrating immune cells in archived, whole tumor resections from 94 patients. We characterized these sections by immune-histochemistry using 12 markers and enumerated tumor-infiltrating immune cells at the invasive margins (IM) and centers of tumors (CT). We observed that a higher density of CD3 + cells in the IM and CT was associated with smaller tumor size (T1-T2 stage). Fewer CD3 + cells was associated with larger tumor size (T3-T4 stage). High infiltration of CD3 + and CD8 + cells in IM and CT as well as high CD4 + cell infiltrates in the IM was significantly associated with the absence of lymph node metastases. High infiltrates of CD3 + and CD8 + cells in CT was associated with significantly improved survival. Our results illustrate that the densities and spatial distribution of CD3 + and CD8 + cell infiltrates in primary GBOSCC tumors is predictive of disease progression and survival. Based on our findings, we recommend incorporating immune cell quantification in the TNM classification and routine histopathology reporting of GBOSCC. Immune cell quantification in CT and IM may help predict the efficacy of future therapies.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0242058
DOI:
10.1371/journal.pone.0242058.g001
DOI:
10.1371/journal.pone.0242058.g002
DOI:
10.1371/journal.pone.0242058.g003
DOI:
10.1371/journal.pone.0242058.g004
DOI:
10.1371/journal.pone.0242058.g005
DOI:
10.1371/journal.pone.0242058.t001
DOI:
10.1371/journal.pone.0242058.t002
DOI:
10.1371/journal.pone.0242058.s001
DOI:
10.1371/journal.pone.0242058.s002
DOI:
10.1371/journal.pone.0242058.s003
DOI:
10.1371/journal.pone.0242058.s004
DOI:
10.1371/journal.pone.0242058.s005
DOI:
10.1371/journal.pone.0242058.s006
DOI:
10.1371/journal.pone.0242058.s007
DOI:
10.1371/journal.pone.0242058.s008
DOI:
10.1371/journal.pone.0242058.r001
DOI:
10.1371/journal.pone.0242058.r002
DOI:
10.1371/journal.pone.0242058.r003
DOI:
10.1371/journal.pone.0242058.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2267670-3
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