In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 5 ( 2021-5-25), p. e0250518-
Abstract:
Gestational trophoblastic disease (GTD) is a heterogeneous group of lesions arising from placental tissue. Epithelioid trophoblastic tumor (ETT), derived from chorionic-type trophoblast, is the rarest form of GTD with only approximately 130 cases described in the literature. Due to its morphologic mimicry of epithelioid smooth muscle tumors and carcinoma, ETT can be misdiagnosed. To date, molecular characterization of ETTs is lacking. Furthermore, ETT is difficult to treat when disease spreads beyond the uterus. Here using RNA-Seq analysis in a cohort of ETTs and other gestational trophoblastic lesions we describe the discovery of LPCAT1-TERT fusion transcripts that occur in ETTs and coincide with underlying genomic deletions. Through cell-growth assays we demonstrate that LPCAT1-TERT fusion proteins can positively modulate cell proliferation and therefore may represent future treatment targets. Furthermore, we demonstrate that TERT upregulation appears to be a characteristic of ETTs, even in the absence of LPCAT1-TERT fusions, and that it appears linked to copy number gains of chromosome 5. No evidence of TERT upregulation was identified in other trophoblastic lesions tested, including placental site trophoblastic tumors and placental site nodules, which are thought to be the benign chorionic-type trophoblast counterpart to ETT. These findings indicate that LPCAT1-TERT fusions and copy-number driven TERT activation may represent novel markers for ETT, with the potential to improve the diagnosis, treatment, and outcome for women with this rare form of GTD.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0250518
DOI:
10.1371/journal.pone.0250518.g001
DOI:
10.1371/journal.pone.0250518.g002
DOI:
10.1371/journal.pone.0250518.g003
DOI:
10.1371/journal.pone.0250518.g004
DOI:
10.1371/journal.pone.0250518.g005
DOI:
10.1371/journal.pone.0250518.t001
DOI:
10.1371/journal.pone.0250518.t002
DOI:
10.1371/journal.pone.0250518.t003
DOI:
10.1371/journal.pone.0250518.t004
DOI:
10.1371/journal.pone.0250518.s001
DOI:
10.1371/journal.pone.0250518.s002
DOI:
10.1371/journal.pone.0250518.s003
DOI:
10.1371/journal.pone.0250518.s004
DOI:
10.1371/journal.pone.0250518.s005
DOI:
10.1371/journal.pone.0250518.s006
DOI:
10.1371/journal.pone.0250518.s007
DOI:
10.1371/journal.pone.0250518.s008
DOI:
10.1371/journal.pone.0250518.s009
DOI:
10.1371/journal.pone.0250518.s010
DOI:
10.1371/journal.pone.0250518.s011
DOI:
10.1371/journal.pone.0250518.s012
DOI:
10.1371/journal.pone.0250518.s013
DOI:
10.1371/journal.pone.0250518.s014
DOI:
10.1371/journal.pone.0250518.r001
DOI:
10.1371/journal.pone.0250518.r002
DOI:
10.1371/journal.pone.0250518.r003
DOI:
10.1371/journal.pone.0250518.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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