In:
Journal of the American Society of Nephrology, Ovid Technologies (Wolters Kluwer Health), Vol. 33, No. 11 ( 2022-11), p. 2026-2039
Abstract:
Chronic lesions constitute an independent risk factor for late kidney graft failure. However, there is currently no validated data-driven system to realistically describe the chronic pathology of kidney transplants. The authors describe the application of clustering methods to characterize the chronicity and severity of renal allograft disease, finding that kidney transplant biopsies cluster into four chronic phenotypes, validated on data from an independent cohort. These chronic phenotypes are distributed independently of the acute rejection phenotypes, demonstrating independent histologic information. Although chronicity is time dependent, the pattern of chronic lesions is more important than the timing of the biopsy in predicting graft failure. These findings highlight the need to assess both the acute and the chronic components of a biopsy to provide a holistic view of kidney transplant histology. Background No validated system currently exists to realistically characterize the chronic pathology of kidney transplants that represents the dynamic disease process and spectrum of disease severity. We sought to develop and validate a tool to describe chronicity and severity of renal allograft disease and integrate it with the evaluation of disease activity. Methods The training cohort included 3549 kidney transplant biopsies from an observational cohort of 937 recipients. We reweighted the chronic histologic lesions according to their time-dependent association with graft failure, and performed consensus k -means clustering analysis. Total chronicity was calculated as the sum of the weighted chronic lesion scores, scaled to the unit interval. Results We identified four chronic clusters associated with graft outcome, based on the proportion of ambiguous clustering. The two clusters with the worst survival outcome were determined by interstitial fibrosis and tubular atrophy (IFTA) and by transplant glomerulopathy. The chronic clusters partially overlapped with the existing Banff IFTA classification (adjusted Rand index, 0.35) and were distributed independently of the acute lesions. Total chronicity strongly associated with graft failure (hazard ratio [HR], 8.33; 95% confidence interval [CI] , 5.94 to 10.88; P 〈 0.001), independent of the total activity scores (HR, 5.01; 95% CI, 2.83 to 7.00; P 〈 0.001). These results were validated on an external cohort of 4031 biopsies from 2054 kidney transplant recipients. Conclusions The evaluation of total chronicity provides information on kidney transplant pathology that complements the estimation of disease activity from acute lesion scores. Use of the data-driven algorithm used in this study, called RejectClass, may provide a holistic and quantitative assessment of kidney transplant injury phenotypes and severity.
Type of Medium:
Online Resource
ISSN:
1046-6673
,
1533-3450
DOI:
10.1681/ASN.2022030290
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2022
detail.hit.zdb_id:
2029124-3
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