In:
FEBS Open Bio, Wiley, Vol. 9, No. 7 ( 2019-07), p. 1184-1193
Abstract:
Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae ( yVDAC 2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC 2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC 2 isolation, through heterologous expression in bacteria and refolding in vitro . Recombinant yVDAC 2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC 2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells.
Type of Medium:
Online Resource
ISSN:
2211-5463
,
2211-5463
DOI:
10.1002/feb4.2019.9.issue-7
DOI:
10.1002/2211-5463.12574
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2651702-4
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