In:
Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 106, No. 12_suppl_1 ( 2002-09-24)
Abstract:
Background Surgical therapy for aortic stenosis leads to reverse remodeling, with normalization of left ventricular hypertrophy (LVH). The aim of this study was to examine Renin-Angiotensin system (RAS) gene expression in this setting. Methods Growing sheep (n=44) underwent supracoronary aortic banding for controlled induction of LVH at the age of 6 to 8 months (A=baseline). Surgical revision to completely release the pressure gradient was performed 8.3±1 months later (B). The animals were sacrificed after another 10.1±2 months (C). Along with hemodynamic measurements, subtractive hybridization and competitive polymerase chain reaction were applied to quantify mRNA expression for angiotensin-converting enzyme (ACE) and angiotensin receptors 1 and 2 (AT 1 -R and AT 2 -R). Results Left ventricular mass index was 82±21 g (A), 150±33 g (B), and 78±18 g (C), P 〈 0.01. Left ventricular function and cardiac index remained stable. Myocardial fiber diameter was 11.3±0.8 (A), 15.9±1.2 (B), and 11.4±1 (C) μm, P 〈 0.01. Gene expression was as follows: ACE 0.8±0.05 (A), 1.3±0.08 (B), and 0.9±0.06 (C), P 〈 0.01; AT 1 -R 0.7±0.06 (A), 0.9±0.07 (B), and 0.3±0.04 (C), P 〈 0.01; AT 2 -R 0.5±0.05 (A), 0.2±0.04 (B), and 0.5±0.05 (C), P 〈 0.01. Conclusion LVH in aortic stenosis coincides with significant alterations of the RAS. Surgical therapy leads to reverse remodeling, which is paralleled by regression of RAS gene expression.
Type of Medium:
Online Resource
ISSN:
0009-7322
,
1524-4539
DOI:
10.1161/01.cir.0000032919.33237.4d
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2002
detail.hit.zdb_id:
1466401-X
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