In:
Proceedings of the Royal Society B: Biological Sciences, The Royal Society, Vol. 283, No. 1825 ( 2016-02-24), p. 20160042-
Abstract:
RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus , and a short-lived globally invasive polyphagous agricultural pest, western flower thrips ( Frankliniella occidentalis ). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were horizontally transmissible. This represents a significant advance in the ability to deliver RNAi, potentially to a large range of non-model insects.
Type of Medium:
Online Resource
ISSN:
0962-8452
,
1471-2954
DOI:
10.1098/rspb.2016.0042
Language:
English
Publisher:
The Royal Society
Publication Date:
2016
detail.hit.zdb_id:
1460975-7
SSG:
12
SSG:
25
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