In:
Open Access Macedonian Journal of Medical Sciences, Scientific Foundation SPIROSKI, Vol. 5, No. 5 ( 2017-08-05), p. 578-586
Abstract:
AIM: Here we imposed a multimarker molecular panel composed of P53, MDM2 protein & mRNA & P16 with the identification of sensitive and specific cut offs among the Egyptian urothelial carcinomas bilharzial or not emphasize the pathological and molecular classifications, pathways and prognosis as a privilege for adjuvant therapy.METHODS: Three hundred and ten urothelial lesions were pathologically evaluated and grouped as follows: 50 chronic cystitis as benign, 240 urothelial carcinomas and 20 normal bladder tissue as a control. Immunohistochemistry for MDM Protein, P16 & p53 and In Situ Hybridization for MDM2mRNA were done.RESULTS: MDM2mRNA overexpression correlated with low grade low stage non invasive tumors, while P53 〉 40% & p16 〈 10% cut offs correlated with high grade high stage invasive carcinomas & bilharzial tumors (P=0.000).CONCLUSION: MDM2mRNA overexpression vs. P53 〉 40% & P16 〈 10% constitutes a multimarker molecular panel with significant cut offs, proved to distinguish low grade, low stage non invasive urothelial carcinomas (MDM2mRNA overexpression, P53 〈 40%, P16 〉 10%) from high grade, high stage invasive urothelial carcinomas (with p53 〉 40, p16 〈 10% & absent MDM2mRNA overexpression). Combined P53 〉 40 & p16 〈 10%, together with the histopathological features can distinguish in situ urothelial lesions from dysplastic and atypical lesions.
Type of Medium:
Online Resource
ISSN:
1857-9655
DOI:
10.3889/oamjms.2017.100
Language:
Unknown
Publisher:
Scientific Foundation SPIROSKI
Publication Date:
2017
detail.hit.zdb_id:
3032255-8
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