In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 4 ( 2022-4-4), p. e0266391-
Abstract:
Duchenne muscular dystrophy (DMD) is an intractable genetic muscular disorder characterized by the loss of DYSTROPHIN. The restoration of DYSTROPHIN is expected to be a curative therapy for DMD. Because muscle stem cells (MuSCs) can regenerate damaged myofibers with full-length DYSTROPHIN in vivo , their transplantation is being explored as such a therapy. As for the transplanted cells, primary satellite cells have been considered, but donor shortage limits their clinical application. We previously developed a protocol that differentiates induced pluripotent stem cells (iPSCs) to MuSCs (iMuSCs). To ameliorate the respiratory function of DMD patients, cell transplantation to the diaphragm is necessary but difficult, because the diaphragm is thin and rapidly moves. In the present study, we explored the transplantation of iMuSCs into the diaphragm. First, we show direct cell injection into the diaphragm of mouse was feasible. Then, to enhance the engraftment of the transplanted cells in a rapidly moving diaphragm, we mixed polymer solutions of hyaluronic acid, alginate and gelatin to the cell suspension, finding a solution of 20% dissolved hyaluronic acid and 80% dissolved gelatin improved the engraftment. Thus, we established a method for cell transplantation into mouse diaphragm and show that an injectable hyaluronic acid-gelatin solution enables the engraftment of iMuSCs in the diaphragm.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0266391
DOI:
10.1371/journal.pone.0266391.g001
DOI:
10.1371/journal.pone.0266391.g002
DOI:
10.1371/journal.pone.0266391.g003
DOI:
10.1371/journal.pone.0266391.g004
DOI:
10.1371/journal.pone.0266391.g005
DOI:
10.1371/journal.pone.0266391.s001
DOI:
10.1371/journal.pone.0266391.s002
DOI:
10.1371/journal.pone.0266391.s003
DOI:
10.1371/journal.pone.0266391.s004
DOI:
10.1371/journal.pone.0266391.s005
DOI:
10.1371/journal.pone.0266391.r001
DOI:
10.1371/journal.pone.0266391.r002
DOI:
10.1371/journal.pone.0266391.r003
DOI:
10.1371/journal.pone.0266391.r004
DOI:
10.1371/journal.pone.0266391.r005
DOI:
10.1371/journal.pone.0266391.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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