In:
Skin Pharmacology and Physiology, S. Karger AG, Vol. 8, No. 4 ( 1995), p. 167-172
Abstract:
Cellular retinoic acid binding proteins (CRABP) are cytoplasmic proteins that bind all- 〈 i 〉 trans 〈 /i 〉 -retinoic acid (RA) and other retinoids. The purpose of these studies was to determine the effects of topically applied RA on CRABP expression in rhino mouse skin. CRABP-II mRNA was significantly induced (3-to 4.5-fold) by a single dose of RA at 6 and 16 h after RA treatment, with a return to control levels at 48 h. CRABP-II message was not significantly elevated by 3 or 4 consecutive days of RA treatment, when assessed 24 h after the last treatment. CRABP-I mRNA was undetectable in control and RA-treated skin. We used radiolabelled RA binding combined with non-denaturing PAGE blot autoradiography to distinguish the CRABP subtypes. By this protein assay method, increases in CRABP-II were detected 24 and 48 h after a single application of RA, as well as after 3 and 4 days of RA treatment. RA treatment did not alter CRABP-I expression relative to the vehicle control. These results demonstrate that in mouse skin CRABP-II, but not CRABP-I, is inducible by RA, and is similar to how RA regulates CRABP in human skin.
Type of Medium:
Online Resource
ISSN:
1660-5527
,
1660-5535
Language:
English
Publisher:
S. Karger AG
Publication Date:
1995
detail.hit.zdb_id:
1483572-1
SSG:
15,3
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