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  • 1
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1969
    In:  Canadian Journal of Zoology Vol. 47, No. 1 ( 1969-01-01), p. 89-94
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 47, No. 1 ( 1969-01-01), p. 89-94
    Abstract: Sections of thymuses from rabbits aged 3 days, 2–3 weeks, and 4 weeks, from young adults (normal and parasitized with coccidiosis), and from old adults were examined for the distribution of eosinophils and heterophils. Developmental and mature stages or lobated and mononuclear eosinophils and lobated heterophils were found. The two lobated granulocytic series, showing transformational stages from myelocyte (macromyelocyte) to lobed forms, as in the bone marrow, were present in the connective tissue of the capsule, interlobular septa, and trabeculae, in the peripheral zone of the cortex, and in the medulla. They were usually found in nests of cells which occasionally showed mitosis. The mononuclear eosinophils, the micromyelocytic line, were found almost exclusively in the medulla, never in nests nor in mitosis. The association of granulocytes with Hassall's corpuscles was variable; granulocytes were seen within some corpuscles, in the vicinity of others, or were not present near others. Thymuses from all rabbits showed granulocytes. The thymuses of the 3-day-old rabbits contained the least concentration of lobed granulocytes and no or very few mononuclear eosinophils. Greatest concentrations of granulocytes were found in old rabbits and parasitized animals. Some possible functions for granulocytes in the thymus are presented.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1969
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1969
    In:  Canadian Journal of Zoology Vol. 47, No. 6 ( 1969-11-01), p. 1381-1387
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 47, No. 6 ( 1969-11-01), p. 1381-1387
    Abstract: Eosinopoiesis was studied in imprints and sections of thymuses of rabbits, aged from 3 days to old adulthood. Two distinctly different morphological lines of development were found, one giving rise to the lobed "blood" eosinophils and another to the mononuclear "tissue" eosinophils. The mononuclear line is believed to arise from the medium-sized thymocytes while the lobed line develops from myeloblasts in the same way as do the bone marrow eosinophils. There is no transformation of mature mononucleated to lobed eosinophils, and mitoses are rarely observed. The thymus of rabbits possesses a noteworthy potential for granulocyte production which is retained as a normal function into late adulthood.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1969
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1969
    In:  Canadian Journal of Zoology Vol. 47, No. 6 ( 1969-11-01), p. 1414-1416
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 47, No. 6 ( 1969-11-01), p. 1414-1416
    Abstract: For the demonstration or intranuclear vesicles, iodine vapor, formalin vapor, and methyl alcohol fixations give equivalent results when followed by staining with the May-Grünwald Giemsa technique. Neutral buffered formalin and May-Grünwald Giemsa staining demonstrate fewer intranuclear vesicles than the first-mentioned combinations. Iodine vapor or formalin vapor fixations followed by Mayer's haematoxylin staining show greater numbers of nuclei with vesicles.Formalin fixes nuclei slowly, which allows nuclear contraction and thus the squeezing-out of vesicles. Haematoxylin stains protein, while May-Grünwald Giemsa technique does not stain proteins but deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It therefore follows that the demonstration of vesicles in blast cell nuclei depends on the fixatives and staining methods used.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1969
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1969
    In:  Canadian Journal of Zoology Vol. 47, No. 6 ( 1969-11-01), p. 1355-1362
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 47, No. 6 ( 1969-11-01), p. 1355-1362
    Abstract: Counts of granulocytes were performed on thymic imprints from normal rabbits 3 days old, 2–3 weeks old, and young adults, and from young adult rabbits mildly and severely parasitized with coccidiosis. Analyses of percentages of eosinophils, heterophils, and basophils indicated no sex differences. Comparisons of relative numbers of granulocytes in normal rabbits showed that young adult rabbits possessed more eosinophils and fewer basophils than the younger groups (no differences in heterophils); 3-day-old rabbits had more eosinophils than the 2-to 3-week animals (no differences in heterophils and basophils). No differences in relative numbers of the various granulocytes were found in the mildly and severely parasitized rabbits, but there was a decrease in eosinophils in the parasitized rabbits when compared with the young normal adult rabbits. Eosinophilic leukocytes were divided into two groups: the mononuclear "tissue" line and the lobated "blood" line of development. In the 3-day-old rabbits about 95% of eosinophils were of the lobed variety but in the older normal rabbits, the two developmental lines were about equal in numbers. When compared with the normal young adult rabbits, parasitized rabbits have increased percentages of "blood" eosinophils as a result of greater numbers of band and polymorphonuclear cells.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1969
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1970
    In:  Canadian Journal of Zoology Vol. 48, No. 4 ( 1970-07-01), p. 709-716
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 48, No. 4 ( 1970-07-01), p. 709-716
    Abstract: Vesicles and cytoplasmic fragments are found in greatest numbers in the lymph node and spleen and least commonly in the thymus and bone narrow in the rabbit. Vesicles appear to originate by the extrusion of intranuclear and intracytoplasmic vesicles mostly from cells of the lymphoid series. Cytoplasmic fragments formed by the pinching off of cytoplasmic buds of blast and lymphoid cells may be either round, oval, or irregular in shape. Vesicles and cytoplasmic fragments are absent from blood smears and extremely difficult to recognize in sections or in areas of imprints where the cells are closely applied one to another. About one-third of all round to oval "naked" blast cells in imprints of mesenteric lymph nodes contain vesicles ranging to 7 μ in diameter although most are about 1 μ in diameter. Large "naked" nuclei contain more vesicles than expected although the size of the nucleus does not affect the size of the vesicles present. We suggest that chromatin from "naked" nuclei and smaller free chromatin masses may become transferred to free vesicles and this process may function in new cell formation.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1970
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1956
    In:  Canadian Journal of Zoology Vol. 34, No. 6 ( 1956-12-01), p. 707-723
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 34, No. 6 ( 1956-12-01), p. 707-723
    Abstract: This paper records a discovery of basic, partially independent nuclear units that lie within a common nuclear membrane of some vertebrate cells. The cells concerned are found in series from small (1–2 μ) to large (10–15 μ or more). The smallest cells possess one basic nuclear unit, the larger cells have several. The series of cells from small to large represent stages of a life cycle. Certain of the basic nuclear units act as reproductive organelles and certain large cells are parent cells producing new small cells thus closing one life cycle and starting another. The large cells are formed from the small cells through a series of fusions. The basic nuclear units behave similarly to micronuclei, the reproductive organelles in ciliates, but the fusions of the vertebrate cells are permanent, not temporary unions such as conjugation in ciliates.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1956
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1970
    In:  Canadian Journal of Zoology Vol. 48, No. 2 ( 1970-03-01), p. 227-230
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 48, No. 2 ( 1970-03-01), p. 227-230
    Abstract: The development of clone cells as protuberations from nuclei of mature erythrocytes in peripheral blood has been described for seven native New Zealand birds. The percentage of new cells ranged from 38.95 in the kiwi to 11.35 in the yellow-eyed penguin. Blood from a native Canadian bird, the white-throated sparrow, had 29.75% and 25.00% new cells. These results support earlier work.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1970
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1969
    In:  Canadian Journal of Zoology Vol. 47, No. 6 ( 1969-11-01), p. 1269-1273
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 47, No. 6 ( 1969-11-01), p. 1269-1273
    Abstract: A form of erythropoiesis occurring in the peripheral blood of young domestic chickens which differs radically from descriptions in classical hematology has been demonstrated with several staining methods and modern methods of microscopy. New erythrocytes arise directly as nuclear buds from young mature erythrocytes. The new cell is a clone, as it were, of the mother cell and classical mitosis is not involved. Stages of progressive differentiation in clone cells terminate with the mature erythrocyte. The frequency of clone cells in peripheral blood ranges from 3.7% to 6.8%, in sharp contrast to the figure of 0.2% for mitosis that has been reported in the literature.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1969
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1960
    In:  Canadian Journal of Zoology Vol. 38, No. 1 ( 1960-02-01), p. 189-198
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 38, No. 1 ( 1960-02-01), p. 189-198
    Abstract: This paper reports the continued investigations into nuclear behaviors and activities of blood cells.Further proof of the extreme changes in shape of the almost naked nuclei of blast cells and evidence of the motility of such nuclei are presented. Further evidence for the release of intranuclear vesicles which become red blood cells is given. The cyclic nature of the life history of lymphocytes, which was first postulated in 1956 (Engelbert (5)), is again emphasized, also the extreme changes in morphology of the whole nucleus as well as the basic nuclear units which take place during the life history. The nucleus should be regarded as a federated composite structure—a federate autonomy—made up of smaller structures, the basic nuclear units, that each have a certain autonomous potential, which comes into play when they leave the composite federation. The nuclear federation can become very large forming polypoid nuclei. Naked nuclei can form "cytoplasm" by contracting and thus squeezing nucleoplasm out into a rim around the nucleus.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1960
    detail.hit.zdb_id: 1490831-1
    SSG: 12
    Library Location Call Number Volume/Issue/Year Availability
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  • 10
    Online Resource
    Online Resource
    Canadian Science Publishing ; 1962
    In:  Canadian Journal of Zoology Vol. 40, No. 1 ( 1962-01-01), p. 83-86
    In: Canadian Journal of Zoology, Canadian Science Publishing, Vol. 40, No. 1 ( 1962-01-01), p. 83-86
    Abstract: This paper describes and illustrates the behavior of blast cell nuclei recorded by a motion picture camera through a phase contrast microscope. This behavior includes extension and contraction of the nuclei to present the classically accepted patterns of a rounded nucleus at one moment and an elongated often twisted shape a few seconds later. The motion involved is not amoeboid action. The elongated nucleus is not accepted in the literature as a normal pattern of a blast cell nucleus but it obviously should be. After an extension the nucleus contracts again presenting the classical rounded form commonly associated with blast cells.The authors believe that this nuclear behavior is a natural function of the blast cell and that it occurs many times during the life history of this cell. It is especially characteristic of the time when the blast cell is releasing most of its nuclear contents to form free basic nuclear units and other components of the blood. A comparison is made with similar blast cells and their nuclear variations in a Feulgen "gentle squash" preparation which is lightly counterstained with fast green F.C.F.
    Type of Medium: Online Resource
    ISSN: 0008-4301 , 1480-3283
    RVK:
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 1962
    detail.hit.zdb_id: 1490831-1
    SSG: 12
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