In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 61, No. 11 ( 2011-11-01), p. 2589-2594
Abstract:
Strain N35 T was isolated from surface-sterilized wheat roots and is a Gram-negative, aerobic, motile straight rod. Strain N35 T tested oxidase-positive and catalase-negative and grew optimally at pH 7.0, 30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis showed over 97 % sequence similarity to strains of the environmental species Acidovorax delafieldii , A. facilis , A. defluvii , A. temperans , A. caeni and A. soli , as well as Acidovorax valerianellae , A. anthurii and Simplicispira metamorpha . DNA–DNA hybridization between strain N35 T and phylogenetically closely related type strains was 25.3–55.7 %, which clearly separates the strain from these closely related species. Additionally, phenotypic properties, such as substrate metabolism profiles as determined by a Biolog GN2 assay and cell-wall fatty acid profiles, particularly contents of the fatty acids C 16 : 0 , C 16 : 1 ω7 c / t , C 17 : 0 , C 17 : 0 cyclo, C 18 : 0 cyclo and C 19 : 0 cyclo, facilitated the differentiation of the newly isolated strain N35 T from its closest relatives. The isolate underwent phenotypic variation at high frequency in laboratory media. The DNA G+C content was 64.9 mol%. We propose that strain N35 T is classified as a representative of a novel species within the genus Acidovorax, and suggest the name Acidovorax radicis sp. nov. The type strain is strain N35 T ( = DSM 23535 T = LMG 25767 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.025296-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2011
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Bookmarklink