In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 5 ( 2023-5-4), p. e0283231-
Abstract:
Omega-3 (n-3) and omega-6 (n-6) polyunsaturated fatty acids (PUFAs) are essential fatty acids with antagonistic inflammatory functions that play vital roles in metabolic health and immune response. Current commercial swine diets tend to over-supplement with n-6 PUFAs, which may increase the likelihood of developing inflammatory diseases and affect the overall well-being of the animals. However, it is still poorly understood how n-6/n-3 PUFA ratios affect the porcine transcriptome expression and how messenger RNAs (mRNAs) and microRNAs (miRNAs) might regulate biological processes related to PUFA metabolism. On account of this, we selected a total of 20 Iberian × Duroc crossbred pigs with extreme values for n-6/n-3 FA ratio (10 high vs 10 low), and longissimus dorsi muscle samples were used to identify differentially expressed mRNAs and miRNAs. The observed differentially expressed mRNAs were associated to biological pathways related to muscle growth and immunomodulation, while the differentially expressed microRNAs ( ssc-miR-30a-3p , ssc-miR-30e-3p , ssc-miR-15b and ssc-miR-7142-3p ) were correlated to adipogenesis and immunity. Relevant miRNA-to-mRNA regulatory networks were also predicted (i.e., mir15b to ARRDC3 ; mir-7142-3p to METTL21C ), and linked to lipolysis, obesity, myogenesis, and protein degradation. The n-6/n-3 PUFA ratio differences in pig skeletal muscle revealed genes, miRNAs and enriched pathways involved in lipid metabolism, cell proliferation and inflammation.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0283231
DOI:
10.1371/journal.pone.0283231.g001
DOI:
10.1371/journal.pone.0283231.g002
DOI:
10.1371/journal.pone.0283231.g003
DOI:
10.1371/journal.pone.0283231.t001
DOI:
10.1371/journal.pone.0283231.s001
DOI:
10.1371/journal.pone.0283231.s002
DOI:
10.1371/journal.pone.0283231.s003
DOI:
10.1371/journal.pone.0283231.s004
DOI:
10.1371/journal.pone.0283231.s005
DOI:
10.1371/journal.pone.0283231.s006
DOI:
10.1371/journal.pone.0283231.s007
DOI:
10.1371/journal.pone.0283231.s008
DOI:
10.1371/journal.pone.0283231.s009
DOI:
10.1371/journal.pone.0283231.s010
DOI:
10.1371/journal.pone.0283231.s011
DOI:
10.1371/journal.pone.0283231.s012
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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