In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-01-09)
Abstract:
RbpA and CarD are essential transcription regulators in mycobacteria. Mechanistic analyses of promoter open complex (RPo) formation establish that RbpA and CarD cooperatively stimulate formation of an intermediate (RP2) leading to RPo; formation of RP2 is likely a bottleneck step at the majority of mycobacterial promoters. Once RPo forms, CarD also disfavors its isomerization back to RP2. We determined a 2.76 Å-resolution crystal structure of a mycobacterial transcription initiation complex (TIC) with RbpA as well as a CarD/RbpA/TIC model. Both CarD and RbpA bind near the upstream edge of the −10 element where they likely facilitate DNA bending and impede transcription bubble collapse. In vivo studies demonstrate the essential role of RbpA, show the effects of RbpA truncations on transcription and cell physiology, and indicate additional functions for RbpA not evident in vitro. This work provides a framework to understand the control of mycobacterial transcription by RbpA and CarD.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.22520.001
DOI:
10.7554/eLife.22520.002
DOI:
10.7554/eLife.22520.003
DOI:
10.7554/eLife.22520.004
DOI:
10.7554/eLife.22520.005
DOI:
10.7554/eLife.22520.006
DOI:
10.7554/eLife.22520.007
DOI:
10.7554/eLife.22520.008
DOI:
10.7554/eLife.22520.009
DOI:
10.7554/eLife.22520.010
DOI:
10.7554/eLife.22520.011
DOI:
10.7554/eLife.22520.012
DOI:
10.7554/eLife.22520.013
DOI:
10.7554/eLife.22520.014
DOI:
10.7554/eLife.22520.015
DOI:
10.7554/eLife.22520.016
DOI:
10.7554/eLife.22520.017
DOI:
10.7554/eLife.22520.018
DOI:
10.7554/eLife.22520.019
DOI:
10.7554/eLife.22520.020
DOI:
10.7554/eLife.22520.021
DOI:
10.7554/eLife.22520.022
DOI:
10.7554/eLife.22520.023
DOI:
10.7554/eLife.22520.024
DOI:
10.7554/eLife.22520.025
DOI:
10.7554/eLife.22520.026
DOI:
10.7554/eLife.22520.027
DOI:
10.7554/eLife.22520.028
DOI:
10.7554/eLife.22520.029
DOI:
10.7554/eLife.22520.030
DOI:
10.7554/eLife.22520.031
DOI:
10.7554/eLife.22520.032
DOI:
10.7554/eLife.22520.033
DOI:
10.7554/eLife.22520.046
DOI:
10.7554/eLife.22520.047
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
Bookmarklink