In:
Cellular and Molecular Biology, CMB Association, Vol. 68, No. 2 ( 2022-02-28), p. 8-18
Abstract:
Diabetes is associated with reproductive impairment in the male reproductive system and causes
complications such as decreased libido, fertility, spermatogenesis, sperm motility, and morphology. High levels of blood sugar may affect sperm quality and reduce the potential for male fertility. Increased levels
of sperm DNA damage are often associated with reduced count and motility or abnormal morphology. This experimental study was conducted at Mashhad University of Medical Sciences. In this work, 40 mice
(C57BL/6) were divided randomly into 4 groups: 1) Control, 2) Diabetic, 3) Diabetic + Insulin, and 4) Sham. After 35 days, the right epididymis of all specimens was used for Real-Time PCR and the left
epididymis for evaluation of sperm parameters using Aniline blue, Toluidine blue, Papanicolaou, and immunohistochemical study. Also, testes were applied for immunohistochemical, TUNEL studies, and
biochemical assay. Results of this study showed that chromatin integrity, morphology, cation channels of sperm (Catsper) expression, and biochemical factors level were significantly changed in diabetic mice
in comparison to other groups (P 〈 0.05) and treatment with insulin improved these parameters. Our
findings showed that the sperm parameters such as DNA integrity, morphology, and Catsper expression change in diabetic mice.
Type of Medium:
Online Resource
ISSN:
1165-158X
,
0145-5680
DOI:
10.14715/cmb/2022.68.2.2
Language:
Unknown
Publisher:
CMB Association
Publication Date:
2022
detail.hit.zdb_id:
2161289-4
SSG:
12
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