In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 9 ( 2022-9-6), p. e3001772-
Abstract:
Potassium ion (K + ) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K + biosensors are promising tools to further improve our understanding of K + -dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K + biosensor, GINKO1, in the K + -bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K + biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K + dynamics in multiple model organisms, including bacteria, plants, and mice.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001772
DOI:
10.1371/journal.pbio.3001772.g001
DOI:
10.1371/journal.pbio.3001772.g002
DOI:
10.1371/journal.pbio.3001772.g003
DOI:
10.1371/journal.pbio.3001772.g004
DOI:
10.1371/journal.pbio.3001772.g005
DOI:
10.1371/journal.pbio.3001772.g006
DOI:
10.1371/journal.pbio.3001772.g007
DOI:
10.1371/journal.pbio.3001772.s001
DOI:
10.1371/journal.pbio.3001772.s002
DOI:
10.1371/journal.pbio.3001772.s003
DOI:
10.1371/journal.pbio.3001772.s004
DOI:
10.1371/journal.pbio.3001772.s005
DOI:
10.1371/journal.pbio.3001772.s006
DOI:
10.1371/journal.pbio.3001772.s007
DOI:
10.1371/journal.pbio.3001772.s008
DOI:
10.1371/journal.pbio.3001772.s009
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10.1371/journal.pbio.3001772.s010
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10.1371/journal.pbio.3001772.s011
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10.1371/journal.pbio.3001772.s012
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10.1371/journal.pbio.3001772.s013
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10.1371/journal.pbio.3001772.s014
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10.1371/journal.pbio.3001772.s015
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10.1371/journal.pbio.3001772.s016
DOI:
10.1371/journal.pbio.3001772.s017
DOI:
10.1371/journal.pbio.3001772.s018
DOI:
10.1371/journal.pbio.3001772.s019
DOI:
10.1371/journal.pbio.3001772.s020
DOI:
10.1371/journal.pbio.3001772.s021
DOI:
10.1371/journal.pbio.3001772.s022
DOI:
10.1371/journal.pbio.3001772.r001
DOI:
10.1371/journal.pbio.3001772.r002
DOI:
10.1371/journal.pbio.3001772.r003
DOI:
10.1371/journal.pbio.3001772.r004
DOI:
10.1371/journal.pbio.3001772.r005
DOI:
10.1371/journal.pbio.3001772.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
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