In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 3 ( 2023-3-8), p. e1011201-
Abstract:
Autophagy plays an important role in the infectious processes of diverse pathogens. For instance, cellular autophagy could be harnessed by viruses to facilitate replication. However, it is still uncertain about the interplay of autophagy and swine acute diarrhea syndrome coronavirus (SADS-CoV) in cells. In this study, we reported that SADS-CoV infection could induce a complete autophagy process both in vitro and in vivo , and an inhibition of autophagy significantly decreased SADS-CoV production, thus suggesting that autophagy facilitated the replication of SADS-CoV. We found that ER stress and its downstream IRE1 pathway were indispensable in the processes of SADS-CoV-induced autophagy. We also demonstrated that IRE1-JNK-Beclin 1 signaling pathway, neither PERK-EIF2S1 nor ATF6 pathways, was essential during SADS-CoV-induced autophagy. Importantly, our work provided the first evidence that expression of SADS-CoV PLP2-TM protein induced autophagy through the IRE1-JNK-Beclin 1 signaling pathway. Furthermore, the interaction of viral PLP2-TM F451-L490 domain and substrate-binding domain of GRP78 was identified to activate the IRE1-JNK-Beclin 1 signaling pathway, and thus resulting in autophagy, and in turn, enhancing SADS-CoV replication. Collectively, these results not only showed that autophagy promoted SADS-CoV replication in cultured cells, but also revealed that the molecular mechanism underlying SADS-CoV-induced autophagy in cells.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011201
DOI:
10.1371/journal.ppat.1011201.g001
DOI:
10.1371/journal.ppat.1011201.g002
DOI:
10.1371/journal.ppat.1011201.g003
DOI:
10.1371/journal.ppat.1011201.g004
DOI:
10.1371/journal.ppat.1011201.g005
DOI:
10.1371/journal.ppat.1011201.g006
DOI:
10.1371/journal.ppat.1011201.g007
DOI:
10.1371/journal.ppat.1011201.g008
DOI:
10.1371/journal.ppat.1011201.g009
DOI:
10.1371/journal.ppat.1011201.g010
DOI:
10.1371/journal.ppat.1011201.g011
DOI:
10.1371/journal.ppat.1011201.t001
DOI:
10.1371/journal.ppat.1011201.s001
DOI:
10.1371/journal.ppat.1011201.s002
DOI:
10.1371/journal.ppat.1011201.s003
DOI:
10.1371/journal.ppat.1011201.s004
DOI:
10.1371/journal.ppat.1011201.s005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2205412-1
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