In:
Archives of Microbiology, Springer Science and Business Media LLC, Vol. 206, No. 1 ( 2024-01)
Abstract:
The composition of the vaginal microbiota is known to be influenced by various factors and to be associated with several disorders affecting women’s health. Although metagenomics is currently a widely used method for studying the human microbiota, it has certain limitations, such as a lack of information on bacterial viability. It is therefore important to use culture-based methods such as culturomics. Here, we used 35 different culture conditions to comprehensively characterize the vaginal bacterial diversity of a single woman's flora. A total of 206 bacterial species, belonging to six phyla (for a little more than half to Firmicutes , followed mainly by Actinobacteria , Bacteroidetes , and Proteobacteria ) and 45 families, and 2 fungal species were cultivated. While several species of lactobacilli have been isolated, a wide variety of other bacteria were also separated, including 65 never reported before in vaginal flora, including a new bacterial species, Porphyromonas vaginalis sp. nov. Extensive culture-based methods are essential to establish a comprehensive, evidence-based repertoire of bacterial viability. If combined with molecular methods, they can provide a much more thorough understanding of the vaginal microbiota and fulfil the unknown part of metagenomic studies.
Type of Medium:
Online Resource
ISSN:
0302-8933
,
1432-072X
DOI:
10.1007/s00203-023-03742-2
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2024
detail.hit.zdb_id:
1458451-7
detail.hit.zdb_id:
477-7
detail.hit.zdb_id:
124824-8
SSG:
12
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