In:
Acta Endocrinologica, Oxford University Press (OUP), Vol. 101, No. 2 ( 1982-10), p. 301-306
Abstract:
The capacity of adult rat testes rendered surgically cryptorchid to metabolize progesterone 1 was investigated at several time intervals (7, 14, 28 and 42 days) after surgery. Testicular tissue was incubated with [ 3 H]progesterone, the steroid metabolites were isolated and purified using paper and thin layer chromatography, and further identified by isotopic dilution and recrystallization to constant 3 H/ 14 C ratio or constant specific activity ( dpm /μmol). A sharp decrease in the accumulation of testosterone, concomitant with a marked increase in accumulation of 17α-hydroxyprogesterone, was observed as early as 7 days after surgery. No further major changes were observed at 14 or 28 days after surgery. However, at 42 days, accumulation of testosterone was substantially increased, while that of 17α-hydroxyprogesterone decreased to trace levels. 20α-Dihydroprogesterone was detected at different time intervals after surgery. The data suggest that cryptorchidism inhibits the activity of 17–20 desmolase for the first 4 weeks after surgery. The activity of this enzyme is restored by the 42nd day and the metabolic pathway between progesterone and testosterone resembles that of an intact rat. The factors responsible for this spontaneous restoration of the biosynthetic pathway are under investigation.
Type of Medium:
Online Resource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/acta.0.1010301
Language:
Unknown
Publisher:
Oxford University Press (OUP)
Publication Date:
1982
detail.hit.zdb_id:
1485160-X
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