In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 4 ( 2021-4-1), p. e3001146-
Abstract:
General anesthesia is characterized by reversible loss of consciousness accompanied by transient amnesia. Yet, long-term memory impairment is an undesirable side effect. How different types of general anesthetics (GAs) affect the hippocampus, a brain region central to memory formation and consolidation, is poorly understood. Using extracellular recordings, chronic 2-photon imaging, and behavioral analysis, we monitor the effects of isoflurane (Iso), medetomidine/midazolam/fentanyl (MMF), and ketamine/xylazine (Keta/Xyl) on network activity and structural spine dynamics in the hippocampal CA1 area of adult mice. GAs robustly reduced spiking activity, decorrelated cellular ensembles, albeit with distinct activity signatures, and altered spine dynamics. CA1 network activity under all 3 anesthetics was different to natural sleep. Iso anesthesia most closely resembled unperturbed activity during wakefulness and sleep, and network alterations recovered more readily than with Keta/Xyl and MMF. Correspondingly, memory consolidation was impaired after exposure to Keta/Xyl and MMF, but not Iso. Thus, different anesthetics distinctly alter hippocampal network dynamics, synaptic connectivity, and memory consolidation, with implications for GA strategy appraisal in animal research and clinical settings.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001146
DOI:
10.1371/journal.pbio.3001146.g001
DOI:
10.1371/journal.pbio.3001146.g002
DOI:
10.1371/journal.pbio.3001146.g003
DOI:
10.1371/journal.pbio.3001146.g004
DOI:
10.1371/journal.pbio.3001146.g005
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10.1371/journal.pbio.3001146.g006
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10.1371/journal.pbio.3001146.g007
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10.1371/journal.pbio.3001146.g008
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10.1371/journal.pbio.3001146.g009
DOI:
10.1371/journal.pbio.3001146.t001
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10.1371/journal.pbio.3001146.s001
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10.1371/journal.pbio.3001146.s002
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10.1371/journal.pbio.3001146.s003
DOI:
10.1371/journal.pbio.3001146.s004
DOI:
10.1371/journal.pbio.3001146.s005
DOI:
10.1371/journal.pbio.3001146.s006
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10.1371/journal.pbio.3001146.s007
DOI:
10.1371/journal.pbio.3001146.s008
DOI:
10.1371/journal.pbio.3001146.s009
DOI:
10.1371/journal.pbio.3001146.s010
DOI:
10.1371/journal.pbio.3001146.s011
DOI:
10.1371/journal.pbio.3001146.s012
DOI:
10.1371/journal.pbio.3001146.s013
DOI:
10.1371/journal.pbio.3001146.s014
DOI:
10.1371/journal.pbio.3001146.s015
DOI:
10.1371/journal.pbio.3001146.r001
DOI:
10.1371/journal.pbio.3001146.r002
DOI:
10.1371/journal.pbio.3001146.r003
DOI:
10.1371/journal.pbio.3001146.r004
DOI:
10.1371/journal.pbio.3001146.r005
DOI:
10.1371/journal.pbio.3001146.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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