In:
PLOS Computational Biology, Public Library of Science (PLoS), Vol. 18, No. 4 ( 2022-4-11), p. e1010051-
Abstract:
Understanding of the pairing statistics in solutions populated by a large number of distinct solute species with mutual interactions is a challenging topic, relevant in modeling the complexity of real biological systems. Here we describe, both experimentally and theoretically, the formation of duplexes in a solution of random-sequence DNA (rsDNA) oligomers of length L = 8, 12, 20 nucleotides. rsDNA solutions are formed by 4 L distinct molecular species, leading to a variety of pairing motifs that depend on sequence complementarity and range from strongly bound, fully paired defectless helices to weakly interacting mismatched duplexes. Experiments and theory coherently combine revealing a hybridization statistics characterized by a prevalence of partially defected duplexes, with a distribution of type and number of pairing errors that depends on temperature. We find that despite the enormous multitude of inter-strand interactions, defectless duplexes are formed, involving a fraction up to 15% of the rsDNA chains at the lowest temperatures. Experiments and theory are limited here to equilibrium conditions.
Type of Medium:
Online Resource
ISSN:
1553-7358
DOI:
10.1371/journal.pcbi.1010051
DOI:
10.1371/journal.pcbi.1010051.g001
DOI:
10.1371/journal.pcbi.1010051.g002
DOI:
10.1371/journal.pcbi.1010051.g003
DOI:
10.1371/journal.pcbi.1010051.g004
DOI:
10.1371/journal.pcbi.1010051.g005
DOI:
10.1371/journal.pcbi.1010051.g006
DOI:
10.1371/journal.pcbi.1010051.g007
DOI:
10.1371/journal.pcbi.1010051.g008
DOI:
10.1371/journal.pcbi.1010051.g009
DOI:
10.1371/journal.pcbi.1010051.s001
DOI:
10.1371/journal.pcbi.1010051.s002
DOI:
10.1371/journal.pcbi.1010051.s003
DOI:
10.1371/journal.pcbi.1010051.s004
DOI:
10.1371/journal.pcbi.1010051.r001
DOI:
10.1371/journal.pcbi.1010051.r002
DOI:
10.1371/journal.pcbi.1010051.r003
DOI:
10.1371/journal.pcbi.1010051.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2193340-6
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