In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-8), p. e0250394-
Abstract:
Plant species from Annonaceae are commonly used in traditional medicine to treat various cancer types. This study aimed to investigate the antiproliferative potential of an alkaloid and acetogenin-rich fraction from the fruit peel of Annona crassiflora in HepG2 cells. A liquid-liquid fractionation was carried out on the ethanol extract of A . crassiflora fruit peel in order to obtain an alkaloid and acetogenin-rich fraction (AF-Ac). Cytotoxicity, proliferation and migration were evaluated in the HepG2 cells, as well as the proliferating cell nuclear antigen (PCNA), vinculin and epidermal growth factor receptor (EGFR) expression. In addition, intracellular Ca 2+ was determined using Fluo4-AM and fluorescence microscopy. First, 9 aporphine alkaloids and 4 acetogenins that had not yet been identified in the fruit peel of A . crassiflora were found in AF-Ac. The treatment with 50 μg/mL AF-Ac reduced HepG2 cell viability, proliferation and migration ( p 〈 0.001), which is in accordance with the reduced expression of PCNA and EGFR levels ( p 〈 0.05). Furthermore, AF-Ac increased intracellular Ca 2+ in the HepG2 cells, mobilizing intracellular calcium stores, which might be involved in the anti-migration and anti-proliferation capacities of AF-Ac. Our results support the growth-inhibitory potential of AF-Ac on HepG2 cells and suggest that this effect is triggered, at least in part, by PCNA and EGFR modulation and mobilization of intracellular Ca 2+ . This study showed biological activities not yet described for A . crassiflora fruit peel, which provide new possibilities for further in vivo studies to assess the antitumoral potential of A . crassiflora , especially its fruit peel.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0250394
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10.1371/journal.pone.0250394.g001
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10.1371/journal.pone.0250394.g002
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10.1371/journal.pone.0250394.g003
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10.1371/journal.pone.0250394.g007
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10.1371/journal.pone.0250394.t001
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10.1371/journal.pone.0250394.s001
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10.1371/journal.pone.0250394.s012
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10.1371/journal.pone.0250394.s013
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10.1371/journal.pone.0250394.s014
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10.1371/journal.pone.0250394.s015
DOI:
10.1371/journal.pone.0250394.s016
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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