In:
American Journal of Reproductive Immunology, Wiley, Vol. 79, No. 3 ( 2018-03)
Abstract:
This study aimed to investigate the association of Th1/Th2 polarity induced by CD1d‐restricted invariant natural killer T (iNKT) cells with pregnancy outcome. Methods Two types of iNKT cell stimulants with different cytokine induction properties, alpha‐galactosylceramide (AGC; Th1‐biased inducer), and a sphingosine‐truncated derivative of AGC (OCH; Th2‐biased inducer) were administered to pregnant mice on day 9.5 post‐coitus (pc), and the incidence of pregnancy loss was evaluated. Serum Th1/Th2 cytokine levels after the iNKT cell stimulations were assessed. Cytokine production from cultured splenocytes following iNKT cell activation was analyzed. Results No fetal loss was observed after OCH administration, in clear contrast with the high frequency of pregnancy loss after AGC exposure. High serum levels of IL‐4 and IL‐10 were detected upon OCH administration, whereas a temporary surge of IFN‐γ was observed after AGC administration. In splenocyte cultures, increases in IL‐4 and IL‐10 were noted after OCH administration, whereas IL‐12 production was enhanced by AGC. Additionally, AGC‐induced pregnancy loss was inhibited by IL‐4 administration. Conclusion The resistance of mouse pregnancy to iNKT cell stimulation by OCH and the prevention of AGC‐induced fetal loss by IL‐4 were demonstrated. In pregnancy, the regulation of Th1/Th2 polarity by iNKT cells is a key to healthy fetal growth.
Type of Medium:
Online Resource
ISSN:
1046-7408
,
1600-0897
DOI:
10.1111/aji.2018.79.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2024667-5
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