In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 3 ( 2021-3-19), p. e1009451-
Abstract:
Salmonella enterica is a diverse bacterial pathogen and a primary cause of human and animal infections. While many S . enterica serovars present a broad host-specificity, several specialized pathotypes have been adapted to colonize and cause disease in one or limited numbers of host species. The underlying mechanisms defining Salmonella host-specificity are far from understood. Here, we present genetic analysis, phenotypic characterization and virulence profiling of a monophasic S . enterica serovar Typhimurium strain that was isolated from several wild sparrows in Israel. Whole genome sequencing and complete assembly of its genome demonstrate a unique genetic signature that includes the integration of the BTP1 prophage, loss of the virulence plasmid, pSLT and pseudogene accumulation in multiple T3SS-2 effectors ( sseJ , steC , gogB , sseK2 , and sseK3 ), catalase ( katE ), tetrathionate respiration ( ttrB ) and several adhesion/ colonization factors ( lpfD , fimH , bigA , ratB , siiC and siiE ) encoded genes. Correspondingly, this strain demonstrates impaired biofilm formation, intolerance to oxidative stress and compromised intracellular replication within non-phagocytic host cells. Moreover, while this strain showed attenuated pathogenicity in the mouse, it was highly virulent and caused an inflammatory disease in an avian host. Overall, our findings demonstrate a unique phenotypic profile and genetic makeup of an overlooked S . Typhimurium sparrow-associated lineage and present distinct genetic signatures that are likely to contribute to its pathoadaptation to passerine birds.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009451
DOI:
10.1371/journal.ppat.1009451.g001
DOI:
10.1371/journal.ppat.1009451.g002
DOI:
10.1371/journal.ppat.1009451.g003
DOI:
10.1371/journal.ppat.1009451.g004
DOI:
10.1371/journal.ppat.1009451.g005
DOI:
10.1371/journal.ppat.1009451.g006
DOI:
10.1371/journal.ppat.1009451.g007
DOI:
10.1371/journal.ppat.1009451.g008
DOI:
10.1371/journal.ppat.1009451.s001
DOI:
10.1371/journal.ppat.1009451.s002
DOI:
10.1371/journal.ppat.1009451.s003
DOI:
10.1371/journal.ppat.1009451.s004
DOI:
10.1371/journal.ppat.1009451.s005
DOI:
10.1371/journal.ppat.1009451.s006
DOI:
10.1371/journal.ppat.1009451.s007
DOI:
10.1371/journal.ppat.1009451.s008
DOI:
10.1371/journal.ppat.1009451.s009
DOI:
10.1371/journal.ppat.1009451.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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