In:
International Journal of Chemical Reactor Engineering, Walter de Gruyter GmbH, Vol. 8, No. 1 ( 2010-01-27)
Abstract:
The secondary structure of the toxin from Bacillus thuringiensis (Bt) is directly correlated with the insecticidal activity and persistence. The conformational changes of Bt toxin before adsorption and after contact with, or adsorbed from, red soil and brown soil were characterized using circular dichroism (CD) and fluorescence spectroscopy. The toxin of Bt contacted with soils for 2 h, the content of ?-helix increased 14.1%-19.0%, and ?-sheet increased 5.1%-5.8%, but the content of the non-regular coil decreased. There was a minor change bout the content of the ?-turns. The fluorescence intensity of contacted toxin decreased and retained 72.8%-93.7% of the intensity of native toxin. The blue-shifts (2.5-3.5 nm) of maximum emission wavelength were observed. The influential trend of desorption was in agreement with the contact course, the influential extent, however, the former was higher than the latter. The content of ?-helix of desorbed toxin increased 33.1%-33.8%, and ?-sheet increased 17.3%-26.8%. The fluorescence intensity retained 70.4%-91.4% and blue-shifts of 3.5 nm were observed.
Type of Medium:
Online Resource
ISSN:
1542-6580
DOI:
10.2202/1542-6580.2136
Language:
Unknown
Publisher:
Walter de Gruyter GmbH
Publication Date:
2010
detail.hit.zdb_id:
2112754-2
Bookmarklink