In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2021 ( 2021-11-2), p. 1-9
Abstract:
Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH), and creatine kinase (CK) levels and cardiomyocyte apoptosis were determined. LDH release, CK activity, caspase-3 activation, mRNA and protein ratio of Bax/Bcl-2, and miR-1 expression were significantly higher ( p 〈 0.01 ) in the H/R model of rat myocardial primary cells and H9c2 cells compared with the control group and was inhibited by YQHX treatment ( p 〈 0.01 or p 〈 0.05 ). We also found that miR-1 overexpression could enhance apoptosis in cardiomyocytes, whereas apoptosis could be reduced by YQHX treatment ( p 〈 0.01 ). In conclusion, YQHX alleviates H/R-induced cardiomyocyte apoptosis by inhibiting miR-1 expression, suggesting the potential of YQHX in preventing MIRI.
Type of Medium:
Online Resource
ISSN:
1741-4288
,
1741-427X
DOI:
10.1155/2021/4852406
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2021
detail.hit.zdb_id:
2148302-4
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