In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 96, No. 6 ( 2006-12), p. 1078-1086
Abstract:
In the present study, the production and fate of bacterial metabolites in the colon were investigated in a direct way using two substrates labelled with stable isotopes: lactose [ 15 N, 15 N]ureide as a source of labelled ammonia and egg proteins intrinsically labelled with [ 2 H 4 ]tyrosine as a precursor of [ 2 H 4 ] p -cresol. Both ammonia and phenolic compounds are believed to be carcinogenic. Stimulation of carbohydrate fermentation in order to prevent accumulation of these toxic metabolites was induced by inclusion of inulin in a test meal or by addition of inulin to the daily diet, allowing us to distinguish between changes induced by the actual presence of a fermentable carbohydrate and effects caused by a long-term dietary intervention. When a single dose of inulin was administered together with the labelled substrates, a significant increase in faecal 15 N excretion, accompanied by a proportional decrease in urinary 15 N excretion was observed, probably reflecting an enhanced uptake of ammonia for bacterial biosynthesis, since an increased concentration of labelled N in bacterial pellets was found. A statistically significant reduction of urinary [ 2 H 4 ] p -cresol excretion was also noted. Upon supplementation of inulin to the daily diet during 4 weeks, however, only a tendency towards decreased urinary excretion of both labelled and unlabelled p -cresol was noted. Further studies are warranted to confirm these results in a larger cohort.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2006
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21
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