In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 89, No. 2-3 ( 1989), p. 301-305
Abstract:
The role played by neutrophils (PMNs) in the genesis of lung injury in diverse clinical situations, such as bronchial asthma, idiopathic pulmonary fibrosis, and the adult respiratory distress syndrome, is an area of intensive investigation. Functional studies of PMNs, particularly those obtained from the alveoli by bronchoalveolar lavage, should shed light on their contribution to lung injury. However, it has not been demonstrated whether procedures used to harvest cells from the lung (bronchoalveolar lavage), particularly the potentially prolonged exposure to saline, commonly used to perform lavage, and other components of lavage fluid, can alter the functional characteristics of PMNs. In this report we demonstrate that a 2- to 3-hour exposure of neutrophils to saline from both humans and sheep in vitro does not alter the functional characteristics of PMNs as determined by superoxide anion generation after activation with phorbol myristate acetate (PMA; 6.96 ± 0.44 vs. 7.60 ± 0.32 nmol O 〈 sub 〉 2 〈 /sub 〉 /250,000 PMNs for control and saline-treated human cells, respectively, after a 45-min incubation with 10 〈 sup 〉 –– 〈 /sup 〉 〈 sup 〉 7 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 PMA, and 4.73 ± 0.30 vs. 4.50 ± 0.42 nmol O 〈 sub 〉 2 〈 /sub 〉 /250,000 PMNs for control and saline-treated sheep cells). In a second series of experiments, we studied the effect of exposure of human PMNs to bronchoalveolar lavage fluid supernatants obtained from normal volunteers on superoxide anion generation by neutrophils. Again, spontaneous (1.27 ± 0.49 vs. 1.65 ± 0.71 nmol O 〈 sub 〉 2 〈 /sub 〉 /200,000 PMNs for control and lavage fluid-treated cells, after a 45-min incubation), PMA-triggered (10 〈 sup 〉 ––6 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 ; 5.67 ± 0.27 vs. 5.18 ± 0.55 nmol O 〈 sub 〉 2 〈 /sub 〉 /200,000 PMNs), and f-met peptide-triggered (10 〈 sup 〉 ––6 〈 /sup 〉 〈 i 〉 M 〈 /i 〉 ; 3.34 ± 0.41 vs. 3.04 ± 0.64 nmol O 〈 sub 〉 2 〈 /sub 〉 /200,000 PMNs) generation of superoxide anion was not affected. Therefore, we conclude that prolonged exposure of neutrophils to either saline or bronchoalveolar lavage fluid supernatants does not alter their capacity to generate superoxide anion and that biochemical studies of neutrophils obtained from the air spaces by bronchoalveolar lavage with saline should provide useful information about the functional characteristics of these cells.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
1989
detail.hit.zdb_id:
1482722-0
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