In:
ZooKeys, Pensoft Publishers, Vol. 830 ( 2019-03-14), p. 75-98
Abstract:
Longidoruspolyae sp. n ., a bisexual nematode species found in the rhizosphere of pear tree ( Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8–9.1 mm; a comparatively long odontostyle (114.0–127.5 μm); a narrow lip region (14.0–15.5 μm), anteriorly flattened and almost continuous with the body profile; pocket-like amphidial pouches long, deeply bilobed, and slightly asymmetrical, a guide ring at 37–42 μm from the anterior end; normal arrangement of pharyngeal glands; and a short bluntly rounded to hemispherical tail. Four juvenile stages identified: the first stage with a digitate tail, and the second and subsequent stages with a bluntly rounded tail. Males have one adcloacal pair and a row of 10 or 11 single ventromedian supplements; spicules 71.0–74.5 μm long. Based on morphometric data, the new species belongs to a group of species spread over Europe ( L.arthensis , L.silvae , L.uroshis ,), Iran ( L.kheirii ), and Syria ( L.pauli ), which share common characters such as amphidial fovea, lip region and tail shapes, similar odontostyle and body length, and similar first-stage juvenile tail shape. Codes for identifying the new species are A5, B2, C34, D3, E3, F45, G12, H1, I2, J1, K7. The phylogenetic analysis based on D2-D3 expansion domains of the rRNA gene revealed that the new species has the closest relationships with L.athesinus from Italy and three unidentified Longidorus spp. from USA ( Longidorus sp. 1, Longidorus sp. 2, and Longidorus sp. 6). New morphometric and molecular data (18S rRNA gene, ITS1-5.8S-ITS2 regions and D2-D3 28S rRNA gene sequences) for three populations of L.pisi from Bulgaria were obtained and variations between populations are discussed.
Type of Medium:
Online Resource
ISSN:
1313-2970
,
1313-2989
DOI:
10.3897/zookeys.830.32188
DOI:
10.3897/zookeys.830.32188.figure1
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10.3897/zookeys.830.32188.figure10
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10.3897/zookeys.830.32188.figure2
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10.3897/zookeys.830.32188.figure6
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10.3897/zookeys.830.32188.figure7
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10.3897/zookeys.830.32188.figure8
DOI:
10.3897/zookeys.830.32188.figure9
Language:
Unknown
Publisher:
Pensoft Publishers
Publication Date:
2019
detail.hit.zdb_id:
2445640-8
SSG:
12
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