In:
International Journal of Laboratory Hematology, Wiley, Vol. 39, No. 4 ( 2017-08), p. 418-422
Abstract:
Activated protein C resistance (APCr) leads to hypercoagulability and is due, often but not exclusively, to Factor V Leiden (FVL). The aim of this study was to assess the ex vivo and in vitro interference of the direct factor Xa inhibitor rivaroxaban (RIV) on a prothrombinase‐based assay for APCr detection. Methods An ex vivo study was performed on fresh plasma samples obtained from 44 subjects with FV wild‐type and seven with FVL heterozygous, all treated with RIV. An in vitro study was performed on 15 plasma samples (six from normal subjects, six from heterozygous, and three from homozygous FVL carriers, all frozen specimens) spiked with RIV. RIV concentration was evaluated using a chromogenic assay, and APCr was evaluated by a prothrombinase‐based assay. Results No significant interference of RIV on APCr results obtained by a prothrombinase‐based assay was observed for drug concentrations up to 400 ng/mL in FV wild‐type and FVL carriers (homozygous and heterozygous). These results were confirmed both ex vivo and in vitro . Conclusions RIV did not significantly interfere with the prothrombinase‐based assay used for the assessment of APCr, and this was observed to occur independently of FV status. However, only concentrations up to 400 ng/mL were tested and, therefore, what occurs in the presence of higher doses remains to be investigated.
Type of Medium:
Online Resource
ISSN:
1751-5521
,
1751-553X
DOI:
10.1111/ijlh.2017.39.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2268600-9
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