In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_2 ( 2013-02-01), p. 412-417
Kurzfassung:
A Gram-staining-negative, rod-shaped, non-spore-forming bacterium isolated as a contaminant from a biopharmaceutical process (strain CCUG 53591 T ) was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence similarity data, this strain was clearly allocated to the genus Herminiimonas . Herminiimonas saxobsidens was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (99.9 %), followed by Herminiimonas glaciei (99.6 %) and Herminiimonas arsenicoxydans (98.8 %). Strain ND5, previously reported as H. glaciei , but showing 100 % 16S rRNA gene sequence similarity to strain CCUG 53591 T , was included in the comparative study. Similarities to all other species of the genus Herminiimonas were below 98.0 %. Chemotaxonomic data (major ubiquinone, Q-8; major polar lipids, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; and major fatty acids, C 17 : 0 cyclo, C 19 : 0 cyclo ω8 c and C 16 : 0, with C 10 : 0 3-OH as hydroxylated fatty acid) supported the affiliation of the isolate to the genus Herminiimonas . DNA–DNA hybridization results (mean values) for strain CCUG 53591 T with H. saxobsidens CCUG 59860 T (34 %), H. glaciei DSM 21140 T (57 %), H. arsenicoxydans DSM 17148 T (17 %) and Herminiimonas fonticola S-94 T (11 %) clearly supported the separate taxonomic position of this strain. Strain ND5 showed DNA–DNA similarities of 78, 56 and 52 % to strain CCUG 53591 T , H. glaciei DSM 21140 T and H. saxobsidens CCUG 59860 T , respectively. Phenotypic differentiation of the isolate from the most closely related species was possible by various features. Hence, strain CCUG 53591 T represents a novel species, for which the name Herminiimonas contaminans sp. nov. is proposed, with the type strain CCUG 53591 T ( = CCM 7991 T ). Strain ND5 is a second strain of this species.
Materialart:
Online-Ressource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.039073-0
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2013
ZDB Id:
215062-1
ZDB Id:
2056611-6
SSG:
12
Bookmarklink