In:
Microscopy and Microanalysis, Oxford University Press (OUP), Vol. 5, No. S2 ( 1999-08), p. 1158-1159
Abstract:
Ultrastructural examination is sometimes necessary for diagnosis of surgical pathology material. Not uncommonly, tissue is not fixed in 3% glutaraldehyde prior to realizing that electron microscopy would be valuable or even necessary to render a diagnosis. It is well known that formalin can be an adequate fixative for ultrastructural evaluation of tissue. Increasingly, non-formalin based fixatives have become more popular with routine surgical pathology practice. Prefer ™ , a glyoxal-based fixative, has become a popular substitute for formalin in many institutions. We are not aware of any study that has compared ultrastructural morphology of tissue fixed in formalin to tissue fixed in a glyoxal-based fixative. To address this question, we took sections of fresh tissue from three different tumors (metastatic melanoma involving a lymph node, squamous cell carcinoma of the lung, and mixed papillary serous and clear cell carcinoma of the ovary) and fixed a portion of each in 3% glutaraldehyde, 10% neutral buffered formalin, and Prefer ™ .
Type of Medium:
Online Resource
ISSN:
1431-9276
,
1435-8115
DOI:
10.1017/S1431927600019115
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1999
detail.hit.zdb_id:
1481716-0
SSG:
11
SSG:
12
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