Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    In: One Health, Elsevier BV, Vol. 10 ( 2020-12), p. 100156-
    Type of Medium: Online Resource
    ISSN: 2352-7714
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2020
    detail.hit.zdb_id: 2834831-X
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    In: Animals, MDPI AG, Vol. 13, No. 19 ( 2023-10-07), p. 3135-
    Abstract: The purpose of this study was twofold: first, to conduct a situational analysis, and second, to assess the knowledge, attitudes, and practices (KAP) of broiler poultry farmers (BPF) regarding antimicrobial use (AMU) and antimicrobial resistance (AMR). Data were collected from 500 BPF across 40 districts, representing 88.1% of Nepal’s broiler poultry population. Among these farmers, 81.0% were male, 59.6% had up to 4 years of experience in poultry farming, and 50.8% had completed at least a high school education. The most used antimicrobials on the farms were doxycycline (23.5%), neomycin (17.1%), and colistin sulfate (9.6%) out of 27 reported antimicrobials. While the BPF exhibited limited knowledge (62.6%) and practice (55.5%) related to AMU and AMR, their overall attitude toward these issues was positive (91.6%). This study also identified significant factors influencing farmers’ attitudes toward AMU and AMR. Farmers aged 31–40 showed a stronger inclination compared to other age groups (OR = 4.2, p = 0.02), and those using antimicrobials for preventive purposes had a more favorable attitude compared to those using them for other purposes (OR = 5.9, p = 0.02). In light of these findings, this study recommends the implementation of effective regulatory measures for drug usage, along with awareness programs addressing AMU and AMR to address the issue of AMR in poultry production.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2606558-7
    SSG: 23
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    In: PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 4 ( 2020-4-2), p. e0223550-
    Type of Medium: Online Resource
    ISSN: 1932-6203
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2020
    detail.hit.zdb_id: 2267670-3
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    In: Animals, MDPI AG, Vol. 10, No. 11 ( 2020-11-07), p. 2060-
    Abstract: Background: Plasmid-mediated resistance to the colistin in poultry is considered as an emerging problem worldwide. While poultry constitutes the major industry in Nepal, there is a paucity of evidence on colistin resistance in Escherichia coli isolates causing natural infections in poultry. This study aimed to explore the prevalence of plasmid-mediated colistin resistance gene, mcr-1 in E. coli isolated from liver samples of dead poultry suspected of E. coli infections. Methods: A total of two hundred and seventy liver samples (227 broilers and 43 layers) from dead poultry suspected of colibacillosis were collected from post-mortem in the Central Veterinary Laboratory (CVL), Kathmandu, between 1 February and 31 July 2019. The specimens were processed to isolate and identify E. coli; an antimicrobial susceptibility test (AST) using disk diffusion method was performed with 12 different antibiotics: Amikacin (30 µg), ampicillin (10 µg), ciprofloxacin (5 µg), chloramphenicol (30 µg), cefoxitin (30 µg), ceftazidime (30 µg), ceftriaxone (30 µg), cotrimoxazole (25 µg), gentamicin (10 µg), imipenem (10 µg), levofloxacin (5 µg) and tetracycline (30 µg). Colistin resistance was determined by agar dilution method and colistin-resistant strains were further screened for plasmid-mediated mcr-1 gene, using conventional polymerase chain reaction (PCR). Results: Out of 270 liver samples, 53.3% (144/270) showed growth of E. coli. The highest number (54%; 109/202) of E. coli isolates was obtained in the liver samples from poultry birds (of both types) aged less than forty days. In AST, 95.1% (137/144) and 82.6% (119/144) of E. coli isolates were resistant against tetracycline and ciprofloxacin, respectively, while 13.2% (19/144) and 25.7% (37/144) isolates were resistant to cefoxitin and imipenem, respectively. In the same assay, 76.4% (110/144) E. coli isolates were multi-drug resistant (MDR). The phenotypic prevalence of colistin resistance was 28.5% (41/144). In the PCR assay, 43.9% (18/41) of colistin-resistant isolates were screened positive for plasmid-mediated mcr-1. Conclusion: The high prevalence of mcr-1 in colistin-resistant E. coli isolates in our study is a cause of concern for the probable coming emergence of colistin resistance in human pathogens, due to horizontal transfer of resistant genes from poultry to human isolates.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2606558-7
    SSG: 23
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    In: Pathogens, MDPI AG, Vol. 12, No. 8 ( 2023-08-15), p. 1045-
    Abstract: Background: Tick-borne protozoan parasites (TBPPs) cause significant problems for domestic animals’ health in Nepal. TBPPs are routinely diagnosed by labor-intensive blood smear microscopy. In Nepal, there are some reports of Babesia and Theileria in cattle, although species identification is rarely performed. Therefore, we performed conventional nested PCR (nPCR) followed by sequence analysis to identify TBPP species infecting cattle in Nepal. Methods: One hundred and six blood samples were collected from cattle in the Kathmandu Valley. Thin blood smears were prepared for microscopic examination. Parasite DNA was extracted from the blood, and nPCR and sequencing were performed to identify the TBPPs present. Results: Among the 106 samples, 45 (42.5%) were positive for piroplasm (Babesia spp. and Theileria spp.) via microscope observation and 56 (52.8%) samples were positive via nPCR. The obtained PCR products were used for direct sequencing, and we identified the species as B. bigemina, B. bovis, T. annulate and T. orientalis. Phylogenetic analyses showed that the B. bovis, B. bigemina and T. orientalis sequences from this study belonged to each species clade. On the other hand, T. annulate was divided into two clades in the analysis, and our T. annulate sequences were also divided in these two clades. The piroplasm-positive cattle showed lower hemoglobin and red blood cells than healthy cattle. Conclusions: To the best of our knowledge, this study is the first to apply molecular detection and species determination of TBPPs in cattle in Nepal. The results of this study may be used as a starting point for the development of successful TBPP surveillance and prevention programs in Nepal.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2695572-6
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Online Resource
    Online Resource
    ZIbeline International Publishing ; 2021
    In:  Malaysian Animal Husbandry Journal Vol. 1, No. 1 ( 2021-09-03), p. 26-32
    In: Malaysian Animal Husbandry Journal, ZIbeline International Publishing, Vol. 1, No. 1 ( 2021-09-03), p. 26-32
    Abstract: The poultry industry has transformed into one of the vibrant economic activities in Nepal, contributing to almost 4% of the national GDP. A cross-sectional survey was conducted among the poultry farmers of Kathmandu valley and Chitwan district to assess the knowledge of antimicrobial resistance (AMR) among the farmers; their practice of antimicrobial use in poultry productions and the biosecurity status of their poultry farms. A semi-structured questionnaire was used to interview a total of 112 respondents, 56 each from the study sites. Nineteen percent (11/56) of the farmers from the Kathmandu valley and 16% (9/56) of that of Chitwan district knew about the antimicrobial resistance (AMR). About 53.6% (30/56) of farmers from Kathmandu valley and 60.7% (34/56) from Chitwan district followed the drug’s withdrawal period. About 33.9% (19/56) of the farmers of Kathmandu valley and 41.1% (23/56) of Chitwan could tell the names of antibiotics they used in their farms. Doxycycline (25.9%, 29/112) was the most used antibiotic followed by Tylosin (21.5%, 24/112), Colistin (18.75%, 21/112), Ciprofloxacin (13.4%, 15/112) and Neomycin (12.5%, 14/112) in both districts. On average of 41.05% (46/112) farmers knew about the harmful effects of drug residues present in the poultry products. 69.6% (39/56) of poultry farms in Kathmandu valley 53.6% (30/56) of Chitwan district had fences. All the farmers of both districts had separate boots for their farms (100%). 8.9% (5/56) of farms in Chitwan district and 16.1% (9/56) of farms in Kathmandu valley disinfected vehicles entering the farms. Nearly 53.6% (30/56) of the farms in Kathmandu valley and 46.4% (26/56) in Chitwan were in close contact with wild birds. Identifying the critical gaps in knowledge and practice of antimicrobials and improving the farms’ biosecurity status could address the issues of AMR in poultry. We suggest a reduction of antimicrobials in the poultry production by adopting alternative measures.
    Type of Medium: Online Resource
    ISSN: 2805-5500
    Language: Unknown
    Publisher: ZIbeline International Publishing
    Publication Date: 2021
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Online Resource
    Online Resource
    Nepal Journals Online (JOL) ; 2020
    In:  International Journal of Applied Sciences and Biotechnology Vol. 8, No. 3 ( 2020-09-29), p. 355-362
    In: International Journal of Applied Sciences and Biotechnology, Nepal Journals Online (JOL), Vol. 8, No. 3 ( 2020-09-29), p. 355-362
    Abstract: In animal products, antibiotic residue is a serious concern for public health in the Himalayan nation, Nepal. A cross-sectional study on antibiotic residues in chicken meat, milk samples of cows, and buffaloes was conducted in selected districts of different provinces of Nepal. Altogether 126 chicken meat samples, 42 each from Kailali (Farwestern Province), Kaski (Gandaki Province), and Nuwakot (Bagmati Province) districts were tested for tetracycline, sulphonamides, penicillin, gentamicin, streptomycin residues by the ELISA method. At Kaski district approximately 57 % (24/42), 50 % (21/42) and 83% (35/42) of meat samples were tested positive for gentamicin (mean-ppb:11.5 μg/Kg/L), tetracycline (mean-ppb:1.44 μg/Kg/L), and fluoroquinolones (mean-ppb: 11.46 μg/ Kg/L) residues respectively. In Kailali and Nuwakot district, 48% (20/42) and 50 % (21/ 42) of meat samples deemed positive for sulphonamides (mean-ppb:15.9 μg/ Kg/L) and penicillin (mean-ppb: 0.39 μg/ Kg/L) residues, respectively. Of the total 168 tests performed in milk samples from Kavre district, 55% (93/168) of the milk samples were tested positive to antibiotic residues for gentamicin, streptomycin, and sulphonamides. The chicken meat samples had antibiotic residues within the national maximum residue limit (MRL); however, milk samples exceeded the national MRL for sulphonamides residues (mean-ppb: 26.44 μg/ Kg/L). The effective surveillance for antibiotic residues in animal products should be implemented strictly in Nepal. Int. J. Appl. Sci. Biotechnol. Vol 8(3): 355-362
    Type of Medium: Online Resource
    ISSN: 2091-2609
    Language: Unknown
    Publisher: Nepal Journals Online (JOL)
    Publication Date: 2020
    detail.hit.zdb_id: 2733295-0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Online Resource
    Online Resource
    Nepal Journals Online (JOL) ; 2022
    In:  International Journal of Applied Sciences and Biotechnology Vol. 10, No. 1 ( 2022-03-29), p. 50-59
    In: International Journal of Applied Sciences and Biotechnology, Nepal Journals Online (JOL), Vol. 10, No. 1 ( 2022-03-29), p. 50-59
    Abstract: The poultry industry has transformed into one of the vibrant economic activities in Nepal, contributing to almost 4% of the national GDP. We conducted a cross-sectional survey among the poultry farmers of Kathmandu valley and Chitwan district to assess their knowledge of antimicrobial resistance (AMR); their practice of antimicrobial usage and the biosecurity status of their poultry farms. The survey was done to understand the level of knowledge gap among the farmers regarding successful poultry farming with strict biosecurity protocol and minimal use of antibiotics. A semi-structured questionnaire was prepared to interview a total of 112 farmers, with 56 respondents from each study sites. Only nineteen percent (11/56) of the farmers from the Kathmandu valley and 16% (9/56) of that of Chitwan district knew about the antimicrobial resistance (AMR). Doxycycline (25.9%, 29/112) was the most used antibiotic followed by Tylosin (21.5%, 24/112), Colistin (18.75%, 21/112), Ciprofloxacin (13.4%, 15/112) and Neomycin (12.5%, 14/112) in both districts. Majority of the surveyed respondents lacked the proper awareness regarding the importance of considerate use of antimicrobial drugs, effectiveness of implementing proper biosecurity protocols in the farms, and the increasing challenge of AMR in the present world scenario. The findings and results of this survey identify the critical gaps in the knowledge of the farmers concerning the day-to-day operations in their poultry business. Immediate strategic actions and road-maps are required to solve the burgeoning problem of AMR considering the imprudent use of antibiotics in the poultry sector. Int. J. Appl. Sci. Biotechnol. Vol 10(1): 50-59.
    Type of Medium: Online Resource
    ISSN: 2091-2609
    Language: Unknown
    Publisher: Nepal Journals Online (JOL)
    Publication Date: 2022
    detail.hit.zdb_id: 2733295-0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2021
    In:  BMC Veterinary Research Vol. 17, No. 1 ( 2021-12)
    In: BMC Veterinary Research, Springer Science and Business Media LLC, Vol. 17, No. 1 ( 2021-12)
    Abstract: Brucellosis is a zoonotic disease caused by Brucella spp. In Nepal, the presence of brucellosis in small ruminants, namely sheep and goats, has impacted farmers’ livelihood and the food safety of consumers. A cross-sectional study was conducted in Rupandehi district of Nepal during January to March 2020 to investigate the seroepidemiology and associated risk factors of brucellosis in the sheep and goat population. Altogether, 19 sheep and 60 goat farms in the district were visited. Owners were interviewed to get information on animals, including their management and movement patterns. Three hundred fifty-seven samples (80 sheep and 277 goat samples) were collected proportionately based on farm sizes. Each serum sample was tested with Rose Bengal Test and ELISA to estimate the seropositivity of brucellosis. Logistic regression was carried out to calculate corresponding odds ratios of each variable associated with detection of brucellosis. Results At the farm level, 31.6% (6/19; 95% CI: 12, 54%) of sheep farms and 3.3% (2/60, 95% CI: 0.9, 11.4%) of goat farms were seropositive to brucellosis. Out of 80 sheep serum samples, 12 (15%; 95% CI: 8.79–24.41%) and out of 277 goat serum samples, three (1.1%; 95% CI: 0.37–3.14%) were seropositive to brucellosis. Age greater than 1.5 years (OR = 5.56, 95% CI: 1.39, 29.38; p  = 0.02) and herd size of greater than 100 (OR = 4.74, 95% CI: 1.23, 20.32, p  = 0.03) were identified as significant risk factors for seropositivity of brucellosis in the sheep population. While in the goat population, none of the variables was identified as a significant risk factor. Conclusion The study provides evidence that the older sheep and the sheep from the large herds were at higher risk of brucellosis. A control program should be put in place immediately in the sheep population because they may transmit infections to other livestock as they were regularly moved for grazing and selling purposes. Also, strict biosecurity measures should be implemented among pastoralists to prevent brucellosis transmission in them. We suggest further one health-based study to reveal the transmission dynamics of brucellosis between animals and humans.
    Type of Medium: Online Resource
    ISSN: 1746-6148
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2191675-5
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    In: Tropical Medicine and Infectious Disease, MDPI AG, Vol. 8, No. 6 ( 2023-05-25), p. 291-
    Abstract: Antimicrobial resistance (AMR) is increasing and represents one of the greatest public health challenges of our time, accounting for considerable morbidity and mortality globally. A “One Health” surveillance strategy, which integrates data concerning the resistant organisms circulating in humans, animals, and the environment, is required to monitor this issue and enable effective interventions. The timely collection, processing, analysis, and reporting of AMR surveillance data are necessary for the effective delivery of the information generated from such surveillance. Nepal has greatly improved its surveillance activities through a network of human and animal health laboratories; however, the data reported by sentinel laboratories are often inconsistent, incomplete, and delayed, causing challenges in terms of data cleaning, standardization, and visualization on a national level. To overcome these issues, innovative methods and procedures have been adopted in Nepal, with the development and customization of digital tools that reduce the human time and effort spent on data cleaning and standardization, with concomitant improvements in the accuracy of data. These standardized data can be uploaded to the district health information system 2 (DHIS2) One Health AMR surveillance portal, enabling the generation of reports that will help decision-makers and policy planners to combat the global problem of AMR.
    Type of Medium: Online Resource
    ISSN: 2414-6366
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2934690-3
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages