In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-17), p. e0237480-
Abstract:
In the absence of a vaccine and other effective prophylactic or therapeutic countermeasures the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) remains a significant public health threat. Attachment and entry of coronaviruses including SARS-CoV-2 is mainly mediated by the spike glycoprotein. Here, we show that iota-carrageenan can inhibit the cell entry of the SARS-CoV-2 spike pseudotyped lentivirus in a dose dependent manner. SARS-CoV-2 spike pseudotyped lentivirus particles were efficiently neutralized with an IC 50 value of 2.6 μg/ml iota-carrageenan. Experiments with patient isolated wild type SARS-CoV-2 virus showed an inhibition of replication in a similar range. In vitro data on iota-carrageenan against various Rhino- and endemic Coronaviruses showed similar IC 50 values and translated readily into clinical effectiveness when a nasal spray containing iota-carrageenan demonstrated a reduction of severity and duration of symptoms of common cold caused by various respiratory viruses. Accordingly, our in vitro data on SARS-CoV-2 spike pseudotyped lentivirus and replication competent SARS-CoV-2 suggest that administration of iota-carrageenan may be an effective and safe prophylaxis or treatment for SARS-CoV-2 infections.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0237480
DOI:
10.1371/journal.pone.0237480.g001
DOI:
10.1371/journal.pone.0237480.g002
DOI:
10.1371/journal.pone.0237480.g003
DOI:
10.1371/journal.pone.0237480.g004
DOI:
10.1371/journal.pone.0237480.g005
DOI:
10.1371/journal.pone.0237480.t001
DOI:
10.1371/journal.pone.0237480.t002
DOI:
10.1371/journal.pone.0237480.s001
DOI:
10.1371/journal.pone.0237480.s002
DOI:
10.1371/journal.pone.0237480.s003
DOI:
10.1371/journal.pone.0237480.s004
DOI:
10.1371/journal.pone.0237480.s005
DOI:
10.1371/journal.pone.0237480.s006
DOI:
10.1371/journal.pone.0237480.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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