In:
Macromolecular Materials and Engineering, Wiley, Vol. 303, No. 6 ( 2018-06)
Abstract:
Development of fluorescent bioprobes for long‐term cell tracking is of great importance to monitor the processes of genesis, development, invasion, and metastasis of cancerous cells. Herein, a new multistimuli‐responsive star polymer of tetraphenylethene‐graft‐tetra‐poly[ N ‐[2‐(diethylamino)‐ethyl]acrylamide] (TPE‐tetraPDEAEAM) with aggregation‐induced emission (AIE) effect for tracing live cells over a long period of time is synthesized by atom transfer radical polymerization using TPE derivative as initiator. TPE‐tetraPDEAEAM gives both lower critical solution temperature and fluorescence responses to the stimulus of the temperature, pH, and CO 2 by combining the thermoresponsive and pH/CO 2 ‐responsive moieties of the diethylamino and acrylamide groups. The AIE‐active TPE‐tetraPDEAEAM has the advantages of very low cytotoxicity, efficient cellular uptake, and strong fluorescence of polymer‐treated cells, which ensure its good performance in long‐term cell tracing. This facile tracking of HeLa cells for as long as nine passages exhibits superior performance in long‐term cell tracing as compared with some commercial cell tracing probes.
Type of Medium:
Online Resource
ISSN:
1438-7492
,
1439-2054
DOI:
10.1002/mame.201700553
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2004372-7
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