In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 2 ( 2022-2-3), p. e1010037-
Abstract:
The benefits of biopesticides and transgenic crops based on the insecticidal Cry-toxins from Bacillus thuringiensis (Bt) are considerably threatened by insect resistance evolution, thus, deciphering the molecular mechanisms underlying insect resistance to Bt products is of great significance to their sustainable utilization. Previously, we have demonstrated that the down-regulation of PxmALP in a strain of Plutella xylostella (L.) highly resistant to the Bt Cry1Ac toxin was due to a hormone-activated MAPK signaling pathway and contributed to the resistance phenotype. However, the underlying transcriptional regulatory mechanism remains enigmatic. Here, we report that the PxGATAd transcription factor (TF) is responsible for the differential expression of PxmALP observed between the Cry1Ac susceptible and resistant strains. We identified that PxGATAd directly activates PxmALP expression via interacting with a non-canonical but specific GATA-like cis -response element (CRE) located in the PxmALP promoter region. A six-nucleotide insertion mutation in this cis -acting element of the PxmALP promoter from the resistant strain resulted in repression of transcriptional activity, affecting the regulatory performance of PxGATAd. Furthermore, silencing of PxGATAd in susceptible larvae reduced the expression of PxmALP and susceptibility to Cry1Ac toxin. Suppressing PxMAP4K4 expression in the resistant larvae transiently recovered both the expression of PxGATAd and PxmALP , indicating that the PxGATAd is a positive responsive factor involved in the activation of PxmALP promoter and negatively regulated by the MAPK signaling pathway. Overall, this study deciphers an intricate regulatory mechanism of PxmALP gene expression and highlights the concurrent involvement of both trans -regulatory factors and cis -acting elements in Cry1Ac resistance development in lepidopteran insects.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010037
DOI:
10.1371/journal.pgen.1010037.g001
DOI:
10.1371/journal.pgen.1010037.g002
DOI:
10.1371/journal.pgen.1010037.g003
DOI:
10.1371/journal.pgen.1010037.g004
DOI:
10.1371/journal.pgen.1010037.g005
DOI:
10.1371/journal.pgen.1010037.g006
DOI:
10.1371/journal.pgen.1010037.g007
DOI:
10.1371/journal.pgen.1010037.g008
DOI:
10.1371/journal.pgen.1010037.g009
DOI:
10.1371/journal.pgen.1010037.s001
DOI:
10.1371/journal.pgen.1010037.s002
DOI:
10.1371/journal.pgen.1010037.s003
DOI:
10.1371/journal.pgen.1010037.s004
DOI:
10.1371/journal.pgen.1010037.s005
DOI:
10.1371/journal.pgen.1010037.s006
DOI:
10.1371/journal.pgen.1010037.s007
DOI:
10.1371/journal.pgen.1010037.s008
DOI:
10.1371/journal.pgen.1010037.s009
DOI:
10.1371/journal.pgen.1010037.s010
DOI:
10.1371/journal.pgen.1010037.s011
DOI:
10.1371/journal.pgen.1010037.r001
DOI:
10.1371/journal.pgen.1010037.r002
DOI:
10.1371/journal.pgen.1010037.r003
DOI:
10.1371/journal.pgen.1010037.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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